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  4. Recombinant Escherichia coli Translational regulator CsrA (csrA)

Recombinant Escherichia coli Translational regulator CsrA (csrA)

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  • 中文名称：
    Recombinant Escherichia coli Translational regulator CsrA(csrA)
  • 货号：
    CSB-EP543019ENTe1
  • 规格：
    ￥3324
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    csrA
  • Uniprot No.：
  • 别名：
    csrA; ECDH10B_2864; Translational regulator CsrA; Carbon storage regulator
  • 种属：
    Escherichia coli (strain K12 / DH10B)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    6.9 kDa
  • 表达区域：
    1-61aa
  • 氨基酸序列
    MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHREEIYQRIQAEKSQQSSY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant E.coli csrA protein is encoded by the gene of csrA (1-61aa). The gene of csrA was cloned in a system (E.coli) that supported the expression of csrA and translation of messenger RNA. Modification of csrA by recombinant DNA technology could lead to the expression of the target protein. The purity is 85% determined by SDS-PAGE.

    CsrA is a protein coding gene that encodes Translational regulator CsrA. According to some research, CsrA may have the following features.
    Global regulation of small RNA binding protein CsrA and non-coding RNA molecule CsrB. Post-transcriptional global regulation of CsrA in bacteria. Under the influence of Escherichia coli global regulator CsrA, the formation and spread of biofilms. The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in E. coli. CsrA regulates the translation of the carbon starvation gene cstA in E. coli by blocking ribosome access to cstA transcripts. The regulatory loops of E. coli CsrA/CsrB and BarA/UvrY systems. Identification and molecular characteristics of the polymorphic gene csrA in Escherichia coli, which affects glycogen biosynthesis, glucose production, cell size and surface characteristics.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Usually binds in the 5'-UTR; binding at or near the Shine-Dalgarno sequence prevents ribosome-binding, repressing translation, binding elsewhere in the 5'-UTR can activate translation and/or stabilize the mRNA. Its function is antagonized by small RNA(s).
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    CsrA/RsmA family
  • 数据库链接：

    KEGG: ecd:ECDH10B_2864