Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli Transcriptional activator rfaH (rfaH)

Recombinant Escherichia coli Transcriptional activator rfaH (rfaH)

  • 中文名称：
    大肠杆菌rfaH重组蛋白
  • 货号：
    CSB-YP365365ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌rfaH重组蛋白
  • 货号：
    CSB-EP365365ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌rfaH重组蛋白
  • 货号：
    CSB-EP365365ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌rfaH重组蛋白
  • 货号：
    CSB-BP365365ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌rfaH重组蛋白
  • 货号：
    CSB-MP365365ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rfaH
  • Uniprot No.：
  • 别名：
    rfaH; hlyT; sfrB; b3842; JW3818; Transcription antitermination protein RfaH
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-162
  • 氨基酸序列
    MQSWYLLYCK RGQLQRAQEH LERQAVNCLA PMITLEKIVR GKRTAVSEPL FPNYLFVEFD PEVIHTTTIN ATRGVSHFVR FGASPAIVPS AVIHQLSVYK PKDIVDPATP YPGDKVIITE GAFEGFQAIF TEPDGEARSM LLLNLINKEI KHSVKNTEFR KL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Enhances distal genes transcription elongation in a specialized subset of operons that encode extracytoplasmic components. RfaH is recruited into a multi-component RNA polymerase complex by the ops element, which is a short conserved DNA sequence located downstream of the main promoter of these operons. Once bound, RfaH suppresses pausing and inhibits Rho-dependent and intrinsic termination at a subset of sites. Termination signals are bypassed, which allows complete synthesis of long RNA chains. Enhances expression of several operons involved in synthesis of lipopolysaccharides, exopolysaccharides, hemolysin, and sex factor. Also negatively controls expression and surface presentation of AG43 and possibly another AG43-independent factor that mediates cell-cell interactions and biofilm formation.
  • 基因功能参考文献：
    1. The presence of the leucine-rich element in the RfaH C-terminal domain may be responsible for its flexible structural behavior. PMID: 25483894
    2. Data indicate that the RfaH transcriptional regulator protein beta-barrel conformation is much more stable than the alpha-helix conformation. PMID: 26033324
    3. The importance of interfacial interactions between the two domains of RfaH is highlighted by the compromised structural integrity of the helical form of the C-terminal doamin in the absence N-terminal domain. PMID: 24758259
    4. These results demonstrate a complex role for RfaH as a regulator of fimB expression via the suppression micA non-coding RNA. PMID: 24163336
    5. The results indicate that all information necessary to control the folding of RfaH is encoded within RfaH itself, whereas accessory factors or sequential folding of N-terminal domain and C-terminal domain during translation are not essential for folding of active RfaH. PMID: 23990324
    6. RfaH ai a transformer protein that uses two alternative folds to carry out two separate functions: autoinhibition of RNAP binding and recruitment of a ribosome. PMID: 23131843
    7. Study shows that the released RfaH-carboxy-terminal domain (CTD) completely refolds from an all-alpha to an all-beta conformation identical to that of NusG-CTD. As a consequence, RfaH-CTD binding to S10 is enabled and translation of RfaH-controlled operons is strongly potentiated. PMID: 22817892
    8. One side of RfaH(N) interacts with the non-template DNA strand during recruitment, whereas a hydrophobic surface on the opposite side of RfaH(N) remains bound to the beta' subunit clamp helices domain throughout transcription of the entire operon. PMID: 20132437
    9. the regulatory effect of RfaH on LPS-core synthesis affects intestinal colonization PMID: 15727837
    10. negatively controls expression and surface presentation of Ag43 and possibly another Ag43-independent factor(s) that mediates cell-cell interactions and biofilm formation. PMID: 16452414
    11. we argue that RfaH binds to the beta' subunit coiled coil, the major target site for the initiation sigma factors. PMID: 17434131

    显示更多

    收起更多

  • 蛋白家族：
    RfaH family
  • 数据库链接：

    KEGG: ecj:JW3818

    STRING: 316385.ECDH10B_4031