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  4. Recombinant Escherichia coli Stringent starvation protein B (sspB)

Recombinant Escherichia coli Stringent starvation protein B (sspB)

  • 中文名称：
    大肠杆菌sspB重组蛋白
  • 货号：
    CSB-YP365382ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌sspB重组蛋白
  • 货号：
    CSB-EP365382ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌sspB重组蛋白
  • 货号：
    CSB-EP365382ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌sspB重组蛋白
  • 货号：
    CSB-BP365382ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌sspB重组蛋白
  • 货号：
    CSB-MP365382ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sspB
  • Uniprot No.：
  • 别名：
    sspB; b3228; JW3197; Stringent starvation protein B; Adapter protein SspB; Specificity-enhancing factor SspB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-165
  • 氨基酸序列
    MDLSQLTPRR PYLLRAFYEW LLDNQLTPHL VVDVTLPGVQ VPMEYARDGQ IVLNIAPRAV GNLELANDEV RFNARFGGIP RQVSVPLAAV LAIYARENGA GTMFEPEAAY DEDTSIMNDE EASADNETVM SVIDGDKPDH DDDTHPDDEP PQPPRGGRPA LRVVK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP365382ENV, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information for all 4 expression hosts?
Also, have any customers reported any activity from this protein by any chance?
Thanks so much!

A:
Thanks for your inquiry.
Recombinant Escherichia coli Stringent starvation protein B(sspB)
CSB-YP365382ENV >> Yeast
CSB-EP365382ENV >> E.coli
CSB-BP365382ENV >> Baculovirus
CSB-MP365382ENV >> Mammalian cell
Expression Region: 1-165aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows: EP, YP, BP, MP: N-terminal 6xHis-tagged.
Sequence:

MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK


Reference: http://www.uniprot.org/uniprot/P0AFZ3
Very nice to receive your inquiry.Sorry, as this is a semi-custom protein, which means we have never expressed any of these proteins before, so also haven't tested the activity, we can't 100% guarantee its activity.
However, as what we provide is the full length protein, and it's purifie under very mild condition, it should be active. In addition, this is an E. coli self protein
we recommend you to purchase a small size of EP protein to have a try, it's very likely to be active.
In addition, we would like to recommend the following protein, the EP protein has already been successfully expressed, and it's supernatant expression. It's very likely to be active.
The main difference between these two proteins is the species, however, their sequence similarity is 100%.
Recombinant Escherichia coli O157:H7 Stringent starvation protein B(sspB)
CSB-YP360347EOD >> Yeast
CSB-EP360347EOD >> E.coli
CSB-BP360347EOD >> Baculovirus
CSB-MP360347EOD >> Mammalian cell
Expression Region:1-165aa; Full length.
Tag information:EP: N-terminal 6xHis-tagged; YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for other expression system: YP, BP, MP:N-terminal 6xHis-tagged
Sequence:

MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK


Reference: http://www.uniprot.org/uniprot/P0AFZ4

靶点详情

  • 功能：
    Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.; Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion.
  • 基因功能参考文献：
    1. The describe an engineered split-adaptor system, in which sspB protein assembly and delivery of substrates to the ClpXP protease depends on a small molecule (rapamycin). PMID: 21866931
    2. results also reveal highly conserved residues in the ClpX-binding peptides of both E. coli SspB and C. crescentus SspBalpha that play no detectable role in ClpX-binding or substrate delivery. PMID: 20014030
    3. properties of the degradation tag and the number of SspB.ClpX tethering interactions are the major factors that determine the extent to which the substrate and ClpX are engaged in ternary delivery complexes PMID: 17317664
    4. ClpXP, together with SspB, is uniquely adapted for degradation of SsrA-tagged proteins and is responsible for the major part of their degradation in vivo PMID: 18550539
  • 蛋白家族：
    SspB family
  • 数据库链接：

    KEGG: ecj:JW3197

    STRING: 316385.ECDH10B_3405