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  4. Recombinant Escherichia coli Small toxic protein tisB (tisB), partial

Recombinant Escherichia coli Small toxic protein tisB (tisB), partial

  • 中文名称：
    大肠杆菌tisB重组蛋白
  • 货号：
    CSB-YP397682ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌tisB重组蛋白
  • 货号：
    CSB-EP397682ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌tisB重组蛋白
  • 货号：
    CSB-EP397682ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌tisB重组蛋白
  • 货号：
    CSB-BP397682ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌tisB重组蛋白
  • 货号：
    CSB-MP397682ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tisB
  • Uniprot No.：
  • 别名：
    tisB; ysdB; b4618; JW3649; Small toxic protein TisB; LexA-regulated protein TisB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Toxic component of a type I toxin-antitoxin (TA) system. Overexpression causes cessation of growth, induces stress-response, a number of membrane protein genes, and leads to cell death. Inhibits ATP synthesis, ATP levels drop drastically quickly after induction. Part of the programmed response to DNA damage; damage leads to increased accumulation of the protein which slows or stops bacterial growth, probably allowing DNA repair before cells continue to grow.
  • 基因功能参考文献：
    1. The RNA antitoxin IstR-1 sets a threshold for TisB-dependent depolarization under DNA-damaging conditions, resulting in two sub-populations: polarized and depolarized cells. PMID: 27997707
    2. TisB forms clusters of narrow pores that are essential for its mechanism of action PMID: 22728134
    3. TisB is an SOS-induced toxin that targets the inner membrane and causes cell death. It does not affect translation or transcription directly. PMID: 18761622
  • 亚细胞定位：
    Cell inner membrane; Single-pass membrane protein.
  • 数据库链接：

    KEGG: eco:b4618

    STRING: 511145.b4618