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  4. Recombinant Escherichia coli Ribonuclease G (rng)

Recombinant Escherichia coli Ribonuclease G (rng)

  • 中文名称：
    大肠杆菌rng重组蛋白
  • 货号：
    CSB-YP364339ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌rng重组蛋白
  • 货号：
    CSB-EP364339ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌rng重组蛋白
  • 货号：
    CSB-EP364339ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌rng重组蛋白
  • 货号：
    CSB-BP364339ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌rng重组蛋白
  • 货号：
    CSB-MP364339ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rng
  • Uniprot No.：
  • 别名：
    rng; cafA; yhdF; b3247; JW3216Ribonuclease G; RNase G; EC 3.1.26.-; Cytoplasmic axial filament protein
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-489
  • 氨基酸序列
    TAELLVNVT PSETRVAYID GGILQEIHIE REARRGIVGN IYKGRVSRVL PGMQAAFVDI GLDKAAFLHA SDIMPHTECV AGEEQKQFTV RDISELVRQG QDLMVQVVKD PLGTKGARLT TDITLPSRYL VFMPGASHVG VSQRIESESE RERLKKVVAE YCDEQGGFII RTAAEGVGEA ELASDAAYLK RVWTKVMERK KRPQTRYQLY GELALAQRVL RDFADAELDR IRVDSRLTYE ALLEFTSEYI PEMTSKLEHY TGRQPIFDLF DVENEIQRAL ERKVELKSGG YLIIDQTEAM TTVDINTGAF VGHRNLDDTI FNTNIEATQA IARQLRLRNL GGIIIIDFID MNNEDHRRRV LHSLEQALSK DRVKTSVNGF SALGLVEMTR KRTRESIEHV LCNECPTCHG RGTVKTVETV CYEIMREIVR VHHAYDSDRF LVYASPAVAE ALKGEESHSL AEVEIFVGKQ VKVQIEPLYN QEQFDVVMM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    An endonuclease that acts in the processing of the 5'-end of precursors of 16S rRNA. It prefers 5'-monophosphorylated over 5'-OH or 5'-triphosphorylated substrates and cleaves single-stranded sites rich in A and U residues; contributes to RNA turnover. 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides for cleavage. The optimal spacing between the 5' end and the scissile phosphate appears to be 6 nucleotides. Any sequence of unpaired nucleotides at the 5'-end is tolerated. Processes the 5'-end precursors of 23S rRNA. Participates in processing of tRNA(Pro) (proK and proM). Also involved in metabolism of some mRNAs. Cells overproducing this protein form chains of cell with cytoplasmic axial filaments. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles, or merely regulate the formation of this structure.; Confers adaptive resistance to aminoglycoside antibiotics through modulation of 16S rRNA processing.
  • 基因功能参考文献：
    1. Escherichia coli RNase G sensing a 5' monophosphate is required in vivo for the normal rapid decay of functional mRNAs. PMID: 18078441
  • 亚细胞定位：
    Cytoplasm. Cell inner membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    RNase E/G family, RNase G subfamily
  • 数据库链接：

    KEGG: ecj:JW3216

    STRING: 316385.ECDH10B_3423