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  4. Recombinant Escherichia coli RNA polymerase sigma factor RpoH (rpoH)

Recombinant Escherichia coli RNA polymerase sigma factor RpoH (rpoH)

  • 货号：
    CSB-YP313777ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP313777ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP313777ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP313777ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoH
  • Uniprot No.：
  • 别名：
    rpoH; fam; hin; htpR; b3461; JW3426; RNA polymerase sigma factor RpoH; Heat shock regulatory protein F33.4; RNA polymerase sigma-32 factor
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-284
  • 氨基酸序列
    MTDKMQSLAL APVGNLDSYI RAANAWPMLS ADEERALAEK LHYHGDLEAA KTLILSHLRF VVHIARNYAG YGLPQADLIQ EGNIGLMKAV RRFNPEVGVR LVSFAVHWIK AEIHEYVLRN WRIVKVATTK AQRKLFFNLR KTKQRLGWFN QDEVEMVARE LGVTSKDVRE MESRMAAQDM TFDLSSDDDS DSQPMAPVLY LQDKSSNFAD GIEDDNWEEQ AANRLTDAMQ GLDERSQDII RARWLDEDNK STLQELADRY GVSAERVRQL EKNAMKKLRA AIEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response.
  • 基因功能参考文献：
    1. ThiS, an ubiquitin-like protein, is covalently attached to delta(32) and at least 27 other proteins, leading to their subsequent degradation by proteases, in a similar manner to the ubiquitin-proteasome system (UPS) in eukaryotes. PMID: 25721662
    2. co-opts the signal recognition particle to regulate protein homeostasis PMID: 24358019
    3. s conclude that oxidative stress triggers the heat shock response in Escherichia coli sigma(32) dependently. PMID: 23585533
    4. N-terminal half of region 2.1 is required for modulating both metabolic stability and the activity of sigma32 PMID: 15516558
    5. First systematic study of the sigma 32 regulon in E. coli including sigma 32 protein level determination gives insight into the complex network regulated by sigma 32 and how it contributes physiological adaptation to changes in the external environment. PMID: 15757896
    6. response protects the cell membrane and responds to its status: Fully 25% of sigma32 regulon members reside in the membrane and alter its functionality; a disproportionate fraction of overexpressed proteins that induce the response are membrane localized PMID: 16818608
    7. Mutants identify a regulatory step downstream of chaperone binding that is required for both inactivation and degradation of sigma32. PMID: 17968012
    8. sigma32-mediated gene expression is a general response to protein insolubility in E. coli PMID: 17992580
    9. it is deduced from these data that there may be a link between sigma(32) regulon activity and cell division events PMID: 19114495
    10. The -10 region in sigma32 is a composite element required for high promoter activity. PMID: 19400791

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, RpoH subfamily
  • 数据库链接：

    KEGG: ecj:JW3426

    STRING: 316385.ECDH10B_3635