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  4. Recombinant Escherichia coli Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp)

Recombinant Escherichia coli Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp)

  • 货号：
    CSB-YP356643ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356643ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356643ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356643ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356643ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tsaD
  • Uniprot No.：
  • 别名：
    tsaD; gcp; ygjD; b3064; JW3036; tRNA N6-adenosine threonylcarbamoyltransferase; EC 2.3.1.234; N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD; tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-337
  • 氨基酸序列
    MRVLGIETSC DETGIAIYDD EKGLLANQLY SQVKLHADYG GVVPELASRD HVRKTVPLIQ AALKESGLTA KDIDAVAYTA GPGLVGALLV GATVGRSLAF AWDVPAIPVH HMEGHLLAPM LEDNPPEFPF VALLVSGGHT QLISVTGIGQ YELLGESIDD AAGEAFDKTA KLLGLDYPGG PLLSKMAAQG TAGRFVFPRP MTDRPGLDFS FSGLKTFAAN TIRDNGTDDQ TRADIARAFE DAVVDTLMIK CKRALDQTGF KRLVMAGGVS ANRTLRAKLA EMMKKRRGEV FYARPEFCTD NGAMIAYAGM VRFKAGATAD LGVSVRPRWP LAELPAA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. May also be involved in the metabolism of glycated proteins, but does not show sialoglycoprotease activity against glycophorin A.
  • 基因功能参考文献：
    1. This study showed that the transcription of the thr and ilv operons in ygjD mutants was increased through the inhibition of transcription attenuation and that dnaG transcription was reduced. PMID: 21873492
    2. Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. PMID: 21619589
    3. Escherichia coli containing a conserved Gcp prevents the accumulation of toxic glycated proteins. PMID: 20824107
    4. Repression of ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli. PMID: 19376873
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    KAE1 / TsaD family
  • 数据库链接：

    KEGG: ecj:JW3036

    STRING: 316385.ECDH10B_3239