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  4. Recombinant Escherichia coli Phage shock protein A (pspA)

Recombinant Escherichia coli Phage shock protein A (pspA)

  • 货号：
    CSB-YP360290ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP360290ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP360290ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP360290ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP360290ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pspA
  • Uniprot No.：
  • 别名：
    pspA; b1304; JW1297; Phage shock protein A
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-222
  • 氨基酸序列
    GIFSRFADI VNANINALLE KAEDPQKLVR LMIQEMEDTL VEVRSTSARA LAEKKQLTRR IEQASAREVE WQEKAELALL KEREDLARAA LIEKQKLTDL IKSLEHEVTL VDDTLARMKK EIGELENKLS ETRARQQALM LRHQAANSSR DVRRQLDSGK LDEAMARFES FERRIDQMEA EAESHSFGKQ KSLDDQFAEL KADDAISEQL AQLKAKMKQD NQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is also required for membrane integrity, efficient translocation and maintenance of the proton motive force.
  • 基因功能参考文献：
    1. Structure and function of Escherichia coli PspA and Synechocystis Vipp1 N-terminal peptides in the membrane stress sensing and mitigation have been described. PMID: 27806910
    2. Escherichia coli PspA has an enhanced sensitivity for curvature elastic (SCE) stress and a higher affinity for the membrane than Synechocystis Vipp1. PMID: 26330516
    3. The discrete spatial distribution and dynamics of PspA effector in lateral membrane regions depend on MreB and RodZ. PMID: 25118250
    4. Multiple signals are needed to induce the Psp response. PMID: 21259006
    5. These results indicate that oligomers of PspA bind to membrane phospholipids and suppress proton leakage. PMID: 17725563
    6. PspA can form hollow spherical or prolate spheroidal particles of about 30-40nm diameter with a scaffold-like arrangement of protein subunits at the surface. PMID: 18789328
    7. PspA and PspG are localized to the cell poles and traffic between both poles. PMID: 19555453

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  • 亚细胞定位：
    Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes at both cell poles and along the length of the cell.
  • 蛋白家族：
    PspA/IM30 family
  • 数据库链接：

    KEGG: ecj:JW1297

    STRING: 316385.ECDH10B_1421