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  4. Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal)

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal)

  • 中文名称：
    大肠杆菌pal重组蛋白
  • 货号：
    CSB-YP364257ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌pal重组蛋白
  • 货号：
    CSB-EP364257ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌pal重组蛋白
  • 货号：
    CSB-BP364257ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌pal重组蛋白
  • 货号：
    CSB-MP364257ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pal
  • Uniprot No.：
  • 别名：
    pal; excC; b0741; JW0731; Peptidoglycan-associated lipoprotein; PAL; Tol-Pal system lipoprotein Pal
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-173
  • 氨基酸序列
    CSSNKNASN DGSEGMLGAG TGMDANGGNG NMSSEEQARL QMQQLQQNNI VYFDLDKYDI RSDFAQMLDA HANFLRSNPS YKVTVEGHAD ERGTPEYNIS LGERRANAVK MYLQGKGVSA DQISIVSYGK EKPAVLGHDE AAYSKNRRAV LVY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. The Tol-Pal system is also required for polar localization of chemoreceptors clusters. The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity.
  • 基因功能参考文献：
    1. Interestingly, Pal is surface exposed in an 'all or nothing' manner, such that most of the cells contain only internal Pal, with fewer cells ( < 20 %) exhibiting surface Pal. PMID: 25808171
    2. role in the pathogenesis of sepsis and imply that physiologically relevant PAL and LPS are shed into serum and act in concert to initiate inflammation in sepsis. PMID: 15717270
    3. absence of TolA and Pal elicits a sustained extracytoplasmic stress response that in turn reduces O-antigen polymerization but does not affect the stability of the Wzy O-antigen polymerase PMID: 15866920
    4. Pal constitutes a dynamic subcomplex of the division apparatus PMID: 17233825
    5. explains how colicins recruit TolB in the bacterial periplasm and highlights a novel binding mechanism for a natively disordered protein PMID: 17375930
    6. We report here that the coexpression of LolA and outer membrane-specific lipoprotein Pal from a very efficient plasmid causes the unusual accumulation of the LolA-Pal complex in the periplasm. PMID: 18029423

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  • 亚细胞定位：
    Cell outer membrane; Lipid-anchor.
  • 蛋白家族：
    OmpA family, Pal subfamily
  • 数据库链接：

    KEGG: ecj:JW0731

    STRING: 316385.ECDH10B_0808