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  4. Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA)

Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA)

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  • 中文名称：
    大肠杆菌O9：H4lolA重组蛋白
  • 货号：
    CSB-YP419575EJF
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP419575EJF could indicate that this peptide derived from Yeast-expressed Escherichia coli O9:H4 (strain HS) lolA.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP419575EJF could indicate that this peptide derived from Yeast-expressed Escherichia coli O9:H4 (strain HS) lolA.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    lolA
  • Uniprot No.：
  • 别名：
    lolA; EcHS_A0996; Outer-membrane lipoprotein carrier protein
  • 种属：
    Escherichia coli O9:H4 (strain HS)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    22.3 kDa
  • 表达区域：
    22-203aa
  • 氨基酸序列
    DAASDLKSRLDKVSSFHASFTQKVTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWLKDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQFSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have some questions about CSB-EP419575EJF Recombinant Escherichia coli O9:H4 (strain HS) Outer-membrane lipoprotein carrier protein.
1.How long does it take to deliver 1mg of protein?
2.What is the concentration of the protein?
3.Do you do any activity check?
4.Could you please provide me with the full sequence including his-tag and potential cleavage sites.

A:
Very nice to receive your inquiry.
1. The lead time for 1mg is 10-15 working days
2. The specific concentration will be determined after production and shown in COA. If customer has special requirement for concentration, please kindly confirm with us in pre-sales and remark in PO.
3. We haven't validated the activity of this protein. This protein is derived from Escherichia coli, which is full length of mature protein expressed in prokaryotic expression system.
Its expression form is supernatant and can maintain its activity in theory, but we haven't performed validation yet.
4. The following is the complete sequence including tag sequence and target protein sequence.

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFRT+DAASDLKSRLDKVSSFHASFTQKVTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWLKDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQFSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK


The sequence in front of "+" is the tag sequence, and the sequence behind of "+" is the target protein sequence.

靶点详情

  • 功能：
    Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane).
  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    LolA family
  • 数据库链接：

    KEGG: ecx:EcHS_A0996