Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli O157:H7 Protease 7 (ompT)

Recombinant Escherichia coli O157:H7 Protease 7 (ompT)

In Stock
  • 中文名称：
    大肠杆菌O157：H7ompT重组蛋白
  • 货号：
    CSB-EP348292EOD(M)
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP348292EOD(M) could indicate that this peptide derived from E.coli-expressed Escherichia coli O157:H7 ompT.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP348292EOD(M) could indicate that this peptide derived from E.coli-expressed Escherichia coli O157:H7 ompT.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ompT
  • Uniprot No.：
  • 别名：
    ompT; Z1931; ECs1663; Protease 7; EC 3.4.23.49; Omptin; Outer membrane protein 3B; Protease A; Protease VII
  • 种属：
    Escherichia coli O157:H7
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    49.5 kDa
  • 表达区域：
    21-317aa
  • 氨基酸序列
    STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVEASDNDEHYDPKGRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRVTNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The cDNA fragment encoding 21-317aa of Escherichia coli O157: H7 Protease 7 (ompT) was fused with an N-terminal 6xHis-SUMO-tag and then expressed in E.coli cells. The product obtained is the Recombinant full-length of mature Mouse Escherichia coli O157: H7 ompT protein. The purity of this protein was assessed by SDS-PAGE electrophoresis and Coomassie blue staining and reached up to 90%. On the reducing SDS-PAGE gel, there presented a molecular mass band of about 53 kDa. The slightly higher result was attributed to glycosylation. This ompT protein was also validated by the LC-MS/MS analysis. In-stock ompT proteins are available now. In addition to acting as an immunogen for specific antibody synthesis, this recombinant ompT protein may be used in the studies of microbiology.

    OmpT is an outer membrane protease of E.coli and plays an important role in resistance to host-generated antimicrobial peptides (AMPs). OmpT rapidly cleaves and inactivates AMPs, including LL‐37 and protamine. AMP inactivation mediated by OmpT supports host colonization by some pathogenic E. coli strains.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.
  • 基因功能参考文献：
    1. The results indicate that EHEC enhances the secretion of OmpT-loaded outer membrane vesicles in coordination with the activation of virulence genes during infection and blocks bacterial cell attack by LL-37. PMID: 28622430
  • 亚细胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Peptidase A26 family
  • 数据库链接：

    KEGG: ece:Z1931

    STRING: 155864.Z1931