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  4. Recombinant Escherichia coli Multifunctional CCA protein (cca)

Recombinant Escherichia coli Multifunctional CCA protein (cca)

  • 中文名称：
    Recombinant Escherichia coli Multifunctional CCA protein(cca),Yeast
  • 货号：
    CSB-YP487523ENM
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Multifunctional CCA protein(cca),Yeast
  • 货号：
    CSB-EP487523ENM
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Multifunctional CCA protein(cca),Yeast
  • 货号：
    CSB-EP487523ENM-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Multifunctional CCA protein(cca),Yeast
  • 货号：
    CSB-BP487523ENM
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Multifunctional CCA protein(cca),Yeast
  • 货号：
    CSB-MP487523ENM
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cca
  • Uniprot No.：
  • 别名：
    cca; EC55989_3471Multifunctional CCA protein [Includes: CCA-adding enzyme; EC 2.7.7.72; CCA tRNA nucleotidyltransferase; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT); 2'-nucleotidase; EC 3.1.3.-); 2',3'-cyclic phosphodiesterase; EC 3.1.4.-); Phosphatase; EC 3.1.3.-)]
  • 种属：
    Escherichia coli (strain 55989 / EAEC)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-412
  • 氨基酸序列
    MKIYLVGGAV RDALLGLPVK DRDWVVVGST PQEMLDAGYQ QVGRDFPVFL HPQTHEEYAL ARTERKSGSG YTGFTCYAAP DVTLEDDLKR RDLTINALAQ DDNGEIIDPY NGLGDLQNRL LRHVSPAFGE DPLRVLRVAR FAARYAHLGF RIADETLTLM REMTHAGELE HLTPERVWKE TESALTTRNP QVFFQVLRDC GALRVLFPEI DALFGVPAPA RWHPEIDTGI HTLMTLSMAA MLSPQVDVRF ATLCHDLGKG LTPPELWPRH HGHGPAGVKL VEQLCQRLRV PNEIRDLARL VAEFHDLIHT FPMLNPKTIV KLFDSIDAWR KPQRVEQLAL TSEADVRGRT GFESADYPQG RWLREAWEVA QSVPTKAVVE AGFKGVEIRE ELTRRRIAAI ASWKEQRCPK PD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases.
  • 蛋白家族：
    TRNA nucleotidyltransferase/poly(A) polymerase family, Bacterial CCA-adding enzyme type 1 subfamily
  • 数据库链接：

    KEGG: eck:EC55989_3471