Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli Macrodomain Ter protein (matP)

Recombinant Escherichia coli Macrodomain Ter protein (matP)

  • 货号：
    CSB-YP359162ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP359162ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP359162ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP359162ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP359162ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    matP
  • Uniprot No.：
  • 别名：
    matP; ycbG; b0956; JW0939Macrodomain Ter protein
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-150
  • 氨基酸序列
    MKYQQLENLE SGWKWKYLVK KHREGELITR YIEASAAQEA VDVLLSLENE PVLVNGWIDK HMNPELVNRM KQTIRARRKR HFNAEHQHTR KKSIDLEFIV WQRLAGLAQR RGKTLSETIV QLIEDAENKE KYANKMSSLK QDLQALLGKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for spatial organization of the terminus region of the chromosome (Ter macrodomain) during the cell cycle. Prevents early segregation of duplicated Ter macrodomains during cell division. Binds specifically to matS, which is a 13 bp signature motif repeated within the Ter macrodomain.
  • 基因功能参考文献：
    1. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle. PMID: 27171414
    2. Data show that the replication terminus attaches to the divisome in a single segment along the perimeter of the cell in a MatP, ZapA and ZapB proteins-dependent manner. PMID: 26762981
    3. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. PMID: 26818444
    4. studies revealing the molecular basis for Terminus-containing (Ter) chromosome condensation by the Ter-specific factor MatP PMID: 23084832
    5. demonstrated that MatP forms dimers that bind a single matS motif PMID: 22684059
    6. The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP (ycbG), DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. PMID: 18984159

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Note=Accumulates in the cell as a discrete focus that colocalizes with the Ter macrodomain.
  • 蛋白家族：
    MatP family
  • 数据库链接：

    KEGG: ecj:JW0939

    STRING: 316385.ECDH10B_1026