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  4. Recombinant Escherichia coli Lon protease (lon)

Recombinant Escherichia coli Lon protease (lon)

  • 中文名称：
    大肠杆菌lon重组蛋白
  • 货号：
    CSB-YP364352ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌lon重组蛋白
  • 货号：
    CSB-EP364352ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌lon重组蛋白
  • 货号：
    CSB-EP364352ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌lon重组蛋白
  • 货号：
    CSB-BP364352ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌lon重组蛋白
  • 货号：
    CSB-MP364352ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lon
  • Uniprot No.：
  • 别名：
    lon; capR; deg; lopA; muc; b0439; JW0429Lon protease; EC 3.4.21.53; ATP-dependent protease La
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-784
  • 氨基酸序列
    NPERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSIRCL EAAMDHDKKI MLVAQKEAST DEPGVNDLFT VGTVASILQM LKLPDGTVKV LVEGLQRARI SALSDNGEHF SAKAEYLESP TIDEREQEVL VRTAISQFEG YIKLNKKIPP EVLTSLNSID DPARLADTIA AHMPLKLADK QSVLEMSDVN ERLEYLMAMM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE LGEMDDAPDE NEALKRKIDA AKMPKEAKEK AEAELQKLKM MSPMSAEATV VRGYIDWMVQ VPWNARSKVK KDLRQAQEIL DTDHYGLERV KDRILEYLAV QSRVNKIKGP ILCLVGPPGV GKTSLGQSIA KATGRKYVRM ALGGVRDEAE IRGHRRTYIG SMPGKLIQKM AKVGVKNPLF LLDEIDKMSS DMRGDPASAL LEVLDPEQNV AFSDHYLEVD YDLSDVMFVA TSNSMNIPAP LLDRMEVIRL SGYTEDEKLN IAKRHLLPKQ IERNALKKGE LTVDDSAIIG IIRYYTREAG VRGLEREISK LCRKAVKQLL LDKSLKHIEI NGDNLHDYLG VQRFDYGRAD NENRVGQVTG LAWTEVGGDL LTIETACVPG KGKLTYTGSL GEVMQESIQA ALTVVRARAE KLGINPDFYE KRDIHVHVPE GATPKDGPSA GIAMCTALVS CLTGNPVRAD VAMTGEITLR GQVLPIGGLK EKLLAAHRGG IKTVLIPFEN KRDLEEIPDN VIADLDIHPV KRIEEVLTLA LQNEPSGMQV VTAK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, UmuD and at least type II antitoxins CcdA, HipB and MazE. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system.
  • 基因功能参考文献：
    1. the ability of Lon to bind DNA is determined by its ATPase domain, that this binding is required for processing protein substrates in nucleoprotein complexes, and that Lon may help regulate DNA replication in response to growth conditions. PMID: 28292931
    2. These mutant rpoB RNA polymerases affect rcsA transcription, but per se are not defective either at rcsA or at cps promoters ande hence lead to increased capsular polysaccharide synthesis in Deltalon strains of Escherichia coli. PMID: 26403574
    3. (p)ppGpp and Lon protease contribute to the robustness of the cell division. PMID: 26644431
    4. Ec-Lon-protease was found to form complexes with the previously obtained thrombin aptamers whose molecules comprise the duplex domains and G-quadruplex region. PMID: 27125023
    5. Role of mutation of alpha-helical domains in the functioning of ATP-dependent Lon protease of Escherichia coli. PMID: 26762095
    6. The participation of HI(CC) domain in formation of the spatial structures of LonA proteases and/or formation of their complexes with DNA is suggested. PMID: 25895363
    7. Here we show that the cysteine residues act as a redox switch of Lon PMID: 25383757
    8. Immature TorA (apoTorA) is degraded in vivo and in vitro by the Lon protease. Lon interacts with apoTorA but not with holoTorA. PMID: 24211448
    9. Lon(E240K) exists almost exclusively as a dodecamer, whereas wild-type Lon equilibrates between hexamers and dodecamers. PMID: 24123818
    10. Results augget that the cleavage of multiple peptide bonds awaits the "almost complete" delivery of all the scissile sites in Lon protease (lambdaN) to the proteolytic site in an ATP-dependent manner. PMID: 23822859
    11. Here, we demonstrate that Lon catalyzes robust unfolding and degradation of circularly permuted variants of GFP PMID: 23359718
    12. Protein unfolding and degradation by the AAA+ Lon protease. PMID: 22162032
    13. CspD is subject to growth-regulated degradation by the Lon protease. PMID: 21435040
    14. These results suggest that Lon interacts with cardiolipin in biological membranes, which may regulate the functions of Lon as a protein-degrading centre in accordance with environmental changes inside cells. PMID: 21436141
    15. The s report that IbpA and IbpB, the sHSPs of Escherichia coli, are substrates for the AAA+ Lon protease. PMID: 20158612
    16. Detailed comparison with the structures of 11 similar domains established the putative location of the nucleotide-binding site in this first fragment of Lon for which a crystal structure has become available. PMID: 15037242
    17. Three dimensional structure of the N-terminal domain of E. coli Lon protease. PMID: 16199667
    18. formation of a complex then enables Lon to degrade free ribosomal proteins PMID: 16495646
    19. results indicate that Lon has a hexameric ring-shaped structure with a central cavity, and that the establishment of this configuration requires Mg(2+), but not ATP PMID: 16511355
    20. We found that the addition of soxbox DNA or RNA polymerase to an in vitro degradation system decreases the rate of SoxS proteolysis by Lon protease. PMID: 16556231
    21. Because of the differing affinities for ATP, we were able to monitor the activities of the high- and low-affinity ATPase sites of lon separately and determine that they were noninteracting. PMID: 16584195
    22. A revised kinetic model is presented for ATPase activity in Lon protease in both the absence and presence of the model peptide substrate PMID: 16981703
    23. We survey some recent developments in the mechanistic characterization of Lon with an emphasis on the utilization of pre-steady-state enzyme kinetic techniques to determine the timing of the ATPase and peptidase activities of the enzyme. PMID: 17216028
    24. We conclude that Lon protease participates directly in the degradation of tmRNA-tagged proteins. PMID: 17616591
    25. Detection and characterization of two ATP-dependent conformational changes in proteolytically inactive Escherichia coli lon mutants PMID: 17975895
    26. Besides many members of the RcsA regulon (which validates our approach as RcsA is a known Lon substrate), many genes of the sigmaS-dependent general stress response were upregulated in the lon mutant. PMID: 18630346
    27. Using synthetic peptides constituting different regions of the endogenous protein substrate lambdaN, the study demonstrates that the proteolytic site of Escherichia coli Lon exhibits a certain level of localized sequence specificity. PMID: 19285157

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase S16 family
  • 数据库链接：

    KEGG: ecj:JW0429

    STRING: 316385.ECDH10B_0395