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  4. Recombinant Escherichia coli Lipoprotein NlpE (nlpE)

Recombinant Escherichia coli Lipoprotein NlpE (nlpE)

  • 中文名称：
    大肠杆菌nlpE重组蛋白
  • 货号：
    CSB-YP331212ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌nlpE重组蛋白
  • 货号：
    CSB-EP331212ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌nlpE重组蛋白
  • 货号：
    CSB-EP331212ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌nlpE重组蛋白
  • 货号：
    CSB-BP331212ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌nlpE重组蛋白
  • 货号：
    CSB-MP331212ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    nlpE
  • Uniprot No.：
  • 别名：
    nlpE; cutF; b0192; JW0188; Lipoprotein NlpE; Copper homeostasis protein CutF
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    21-236
  • 氨基酸序列
    CNNRAEVDTL SPAQAAELKP MPQSWRGVLP CADCEGIETS LFLEKDGTWV MNERYLGARE EPSSFASYGT WARTADKLVL TDSKGEKSYY RAKGDALEML DREGNPIESQ FNYTLEAAQS SLPMTPMTLR GMYFYMADAA TFTDCATGKR FMVANNAELE RSYLAARGHS EKPVLLSVEG HFTLEGNPDT GAPTKVLAPD TAGKFYPNQD CSSLGQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in copper homeostasis, could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation. Functions during envelope stress responses; when overproduced induces degP through the activation of the two-component envelope stress response system CpxA/CpxR. DegP induction seems to require membrane anchoring of this protein. Structural changes and/or interaction of the CXXC motif with its environment may lead to activation of the Cpx stress response.
  • 基因功能参考文献：
    1. Sensing of adhesion signals via NlpE is important for regulation of the expression of the type III secretion system and flagella in enterohemorrhagic Escherichia coli during infection PMID: 26644384
    2. The nonlipidated Cys1Ala mutant of NlpE with a C-terminal His tag from Escherichia coli was constructed, overexpressed and purified. The tetragonal crystals diffracted to 2.8 A resolution and belong to space group P4(3)2(1)2. PMID: 17142903
  • 亚细胞定位：
    Cell outer membrane; Lipid-anchor.
  • 数据库链接：

    KEGG: ecj:JW0188

    STRING: 316385.ECDH10B_0173