Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli K99 fimbrial protein (fanC)

Recombinant Escherichia coli K99 fimbrial protein (fanC)

In Stock
  • 中文名称：
    大肠杆菌fanC重组蛋白
  • 货号：
    CSB-EP322992ENLe1
  • 规格：
    ￥3324
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP322992ENLe1 could indicate that this peptide derived from E.coli-expressed Escherichia coli fanC.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP322992ENLe1 could indicate that this peptide derived from E.coli-expressed Escherichia coli fanC.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    fanC
  • Uniprot No.：
  • 别名：
    fanCK99 fimbrial protein
  • 种属：
    Escherichia coli
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    16.5kDa
  • 表达区域：
    23-181aa
  • 氨基酸序列
    NTGTINFNGKITSATCTIDPEVNGNRTSTIDLGQAAISGHGTVVDFKLKPAPGSNDCLAKTNARIDWSGSMNSLGFNNTASGNTAAKGYHMTLRATNVGNGSGGANINTSFTTAEYTHTSAIQSFNYSAQLKKDDRAPSNGGYKAGVFTTSASFLVTYM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO synthesized the recombinant gene by integrating the Tag-Free sequence into the targeted gene encoding the 23-181aa of the Escherichia coli fanC. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant Escherichia coli fanC protein carrying Tag-Free. The SDS-PAGE assayed the purity of this recombinant fanC protein greater than 90%. This fanC protein migrated along the gel to a band of about 17 kDa molecular weight.

    fanC is a gene providing instruction of making a protein named K99 fimbrial protein in E.coli and belongs to fimbrial protein family. K99 fimbrial protein is the fimbrial type F5 and specifically mediates the attachment of Enterotoxigenic Escherichia coli (ETEC) to mucosal surfaces of calves, lambs, and piglets. ETEC colonize in the intestine by means of specific adhesion factors (fimbriae) and produce en terotoxins resulting in gastric diseases. Production of K99 requires the expression of eight unique proteins: FanA–H. FanC,the major subunit and immunogenic polypeptide of K99 fimbriae is the most highly expressed of the K99 genes.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.; FanC is the main component of the K99 fimbriae.
  • 亚细胞定位：
    Fimbrium.
  • 蛋白家族：
    Fimbrial protein family