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  4. Recombinant Escherichia coli Exopolyphosphatase (ppx)

Recombinant Escherichia coli Exopolyphosphatase (ppx)

In Stock
  • 中文名称：
    大肠杆菌ppx重组蛋白
  • 货号：
    CSB-EP365319ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365319ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) ppx.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365319ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) ppx.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ppx
  • Uniprot No.：
  • 别名：
    ppx; b2502; JW2487; Exopolyphosphatase; ExopolyPase; EC 3.6.1.11; Metaphosphatase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    74.0kDa
  • 表达区域：
    2-513aa
  • 氨基酸序列
    PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The DNA fragment encoding the 2-513aa of the E.coli ppx protein was fused with N-terminal 6xHis-SUMO tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant E.coli ppx protein. The purity of this recombinant ppx protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant ppx protein showed a band on the gel with a molecular weight of approximately 68-91 kDa.

    ppx is a gene encoding a protein named Exopolyphosphatase and belongs GppA/Ppx family. Exopolyphosphatase (PPX),a phosphatase enzyme, was discovered by the lab of Nobel laureate Arthur Kornberg in 1993.The structure of PPX is characterized by the actin-like ATPase domain. This protein catalyzes and releases orthophosphate processively from the ends of the polyP chain which is a polymer composed of a few to hundreds of phosphate residues supporting cell survival in the stationary phase. This enzyme plays important role in the bacterial stringent response.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Degradation of inorganic polyphosphates (polyP). Releases orthophosphate processively from the ends of the polyP chain. Has a strong preference for long-chain polyphosphates and has only weak affinity for smaller size polyP of about 15 residues.
  • 基因功能参考文献：
    1. H(378) is a fundamental gatekeeper for the recognition of long chain polyphosphate PMID: 27424154
  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    GppA/Ppx family
  • 数据库链接：

    KEGG: ecj:JW2487

    STRING: 316385.ECDH10B_2668