Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli Ditrans,polycis-undecaprenyl-diphosphate synthase ( (2E,6E)-farnesyl-diphosphate specific) (ispU)

Recombinant Escherichia coli Ditrans,polycis-undecaprenyl-diphosphate synthase ( (2E,6E)-farnesyl-diphosphate specific) (ispU)

  • 中文名称：
    大肠杆菌ispU重组蛋白
  • 货号：
    CSB-EP350241ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ispU
  • Uniprot No.：
  • 别名：
    ispU; rth; uppS; yaeS; b0174; JW0169; Ditrans,polycis-undecaprenyl-diphosphate synthase; (2E,6E)-farnesyl-diphosphate specific; EC 2.5.1.31; Ditrans,polycis-undecaprenylcistransferase; Undecaprenyl diphosphate synthase; UDS; Undecaprenyl pyrophosphate synthase; UPP synthase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    32.4 kDa
  • 表达区域：
    1-253aa
  • 氨基酸序列
    MMLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKAGAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRHNVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEKVQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDVLWPDFDEQDFEGALNAFANRERRFGGTEPGDETA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Generates ditrans,octacis-undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP) and farnesyl diphosphate (FPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.
  • 基因功能参考文献：
    1. Unlike the sequential mechanism used by trans-prenyltransferases, these data demonstrate E. coli UPPS utilizes the concerted mechanism. PMID: 20828539
    2. L85, L88 or F89 are important for substrate binding but not specificity. The upper portion of the active-site tunnel, where cis double bonds of the product reside, may be critical for determining the final product chain length. PMID: 15447632
    3. undecaprenyl pyrophosphate synthase (UPPs)is crystallized in a new trigonal unit cell with Mg(2+)/isopentenyl pyrophosphates (IPP)/farnesyl thiopyrophosphate bound to the active site PMID: 15788389
  • 蛋白家族：
    UPP synthase family
  • 数据库链接：

    KEGG: ecj:JW0169

    STRING: 316385.ECDH10B_0154