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  4. Recombinant Escherichia coli DNA replication terminus site-binding protein (tus)

Recombinant Escherichia coli DNA replication terminus site-binding protein (tus)

  • 中文名称：
    大肠杆菌tus重组蛋白
  • 货号：
    CSB-YP324547ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌tus重组蛋白
  • 货号：
    CSB-EP324547ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌tus重组蛋白
  • 货号：
    CSB-EP324547ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌tus重组蛋白
  • 货号：
    CSB-BP324547ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌tus重组蛋白
  • 货号：
    CSB-MP324547ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tus
  • Uniprot No.：
  • 别名：
    tus; tau; b1610; JW1602; DNA replication terminus site-binding protein; Ter-binding protein
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-309
  • 氨基酸序列
    MARYDLVDRL NTTFRQMEQE LAIFAAHLEQ HKLLVARVFS LPEVKKEDEH NPLNRIEVKQ HLGNDAQSLA LRHFRHLFIQ QQSENRSSKA AVRLPGVLCY QVDNLSQAAL VSHIQHINKL KTTFEHIVTV ESELPTAARF EWVHRHLPGL ITLNAYRTLT VLHDPATLRF GWANKHIIKN LHRDEVLAQL EKSLKSPRSV APWTREEWQR KLEREYQDIA ALPQNAKLKI KRPVKVQPIA RVWYKGDQKQ VQHACPTPLI ALINRDNGAG VPDVGELLNY DADNVQHRYK PQAQPLRLII PRLHLYVAD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Trans-acting protein required for termination of DNA replication. Binds to DNA replication terminator sequences (terA to terF) to prevent the passage of replication forks. The termination efficiency will be affected by the affinity of this protein for the terminator sequence.
  • 基因功能参考文献：
    1. We demonstrate that the replisome remains stably bound after encountering a Tus-ter complex from the non-permissive direction. Furthermore, the replisome is only transiently blocked, and continues replication beyond the barrier. Additionally, we demonstrate that these barriers affect sister chromosome segregation by visualizing specific chromosomal loci in the presence and absence of the Tus protein. PMID: 27166373
    2. The study presents a model for replication fork arrest by the Escherichia coli Tus-Ter complex. PMID: 26007657
    3. Data suggest that increased negative supercoiling enhances DnaB unwinding activity, thereby reducing the duration of the Tus-DnaB interaction and leading to decreased Tus activity. PMID: 16238625
    4. As the replication fork helicase unwinds one side of the DNA sequence Ter, a conserved cytosine flips out of the duplex and binds to Tus, thereby creating a locked complex that blocks the advancing helicase. PMID: 16950091
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Tus family
  • 数据库链接：

    KEGG: ecj:JW1602

    STRING: 316385.ECDH10B_1743