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  4. Recombinant Escherichia coli DNA-directed RNA polymerase subunit alpha (rpoA)

Recombinant Escherichia coli DNA-directed RNA polymerase subunit alpha (rpoA)

  • 中文名称：
    大肠杆菌rpoA重组蛋白
  • 货号：
    CSB-YP364088ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌rpoA重组蛋白
  • 货号：
    CSB-EP364088ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌rpoA重组蛋白
  • 货号：
    CSB-EP364088ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌rpoA重组蛋白
  • 货号：
    CSB-BP364088ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌rpoA重组蛋白
  • 货号：
    CSB-MP364088ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoA
  • Uniprot No.：
  • 别名：
    rpoA; pez; phs; sez; b3295; JW3257; DNA-directed RNA polymerase subunit alpha; RNAP subunit alpha; EC 2.7.7.6; RNA polymerase subunit alpha; Transcriptase subunit alpha
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-329
  • 氨基酸序列
    MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE IDGVLHEYST KEGVQEDILE ILLNLKGLAV RVQGKDEVIL TLNKSGIGPV TAADITHDGD VEIVKPQHVI CHLTDENASI SMRIKVQRGR GYVPASTRIH SEEDERPIGR LLVDACYSPV ERIAYNVEAA RVEQRTDLDK LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDVRQP EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL TEIKDVLASR GLSLGMRLEN WPPASIADE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.; Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNAP. It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis.
  • 基因功能参考文献：
    1. Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase has been described. PMID: 27729537
    2. Our investigations provide molecular insights for understanding how EctopoI interacts with RNAP, a critical step for preventing hypernegative DNA supercoiling during transcription PMID: 27448274
    3. Ribonucleoprotein particles of bacterial small non-coding RNA IsrA and its interaction with RNA polymerase core may link transcription to mRNA fate. PMID: 26609136
    4. Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator have been described. PMID: 26602687
    5. The s report that the upstream-bound CRP interacts with thesealpha-CTD domains irrespective of whether it up- or downregulates promoter activity. PMID: 22967981
    6. A self-cleaving elastin-like polypeptide (ELP) tag was used to purify the multisubunit Escherichia coli RNA polymerase (RNAP) via a simple, nonchromatographic method. PMID: 20512976
    7. The direct interaction between RNAPalpha and RPL2 was confirmed both in vivo and in vitro by performing coimmunoprecipitation and bacterial two-hybrid experiments. PMID: 20097853
    8. carboxy-terminal domain of the alpha subunit of RNAP (alpha-CTD)contacts MarA at a surface distant from the DNA. PMID: 15458404
    9. Data show that SoxS diverts RNA polymerase alpha from UP-containing promoters to SoxS-activatable promoters PMID: 15465042
    10. Extensive stable DNA wrapping observed previously in the RNAP-promoter open complex at the lambda P(R) promoter is not a general feature of RNAP-promoter open complexes. PMID: 17290289
    11. Contacts between lambda repressor and the C-terminal domain of the RNA polymerase alpha subunit are required for efficient lambda repressor dependent activation of bacteriophage lambda pM promoter. PMID: 17389649

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  • 蛋白家族：
    RNA polymerase alpha chain family
  • 数据库链接：

    KEGG: ecj:JW3257

    STRING: 316385.ECDH10B_3470