Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli Chromosomal replication initiator protein DnaA (dnaA)

Recombinant Escherichia coli Chromosomal replication initiator protein DnaA (dnaA)

  • 中文名称：
    大肠杆菌dnaA重组蛋白
  • 货号：
    CSB-YP365821ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌dnaA重组蛋白
  • 货号：
    CSB-EP365821ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌dnaA重组蛋白
  • 货号：
    CSB-EP365821ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌dnaA重组蛋白
  • 货号：
    CSB-BP365821ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌dnaA重组蛋白
  • 货号：
    CSB-MP365821ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    dnaA
  • Uniprot No.：
  • 别名：
    dnaA; b3702; JW3679Chromosomal replication initiator protein DnaA
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-467
  • 氨基酸序列
    MSLSLWQQCL ARLQDELPAT EFSMWIRPLQ AELSDNTLAL YAPNRFVLDW VRDKYLNNIN GLLTSFCGAD APQLRFEVGT KPVTQTPQAA VTSNVAAPAQ VAQTQPQRAA PSTRSGWDNV PAPAEPTYRS NVNVKHTFDN FVEGKSNQLA RAAARQVADN PGGAYNPLFL YGGTGLGKTH LLHAVGNGIM ARKPNAKVVY MHSERFVQDM VKALQNNAIE EFKRYYRSVD ALLIDDIQFF ANKERSQEEF FHTFNALLEG NQQIILTSDR YPKEINGVED RLKSRFGWGL TVAIEPPELE TRVAILMKKA DENDIRLPGE VAFFIAKRLR SNVRELEGAL NRVIANANFT GRAITIDFVR EALRDLLALQ EKLVTIDNIQ KTVAEYYKIK VADLLSKRRS RSVARPRQMA MALAKELTNH SLPEIGDAFG GRDHTTVLHA CRKIEQLREE SHDIKEDFSN LIRTLSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in the initiation and regulation of chromosomal replication. Binds in an ATP-dependent fashion to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex exactly once per cell cycle. Binds the DnaA box (consensus sequence 5'-TTATC[CA]A[CA]A-3'); subsequent binding of DNA polymerase III subunits leads to replisome formation. The DnaA-ATP form converts to DnaA-ADP; once converted to ADP the protein cannot initiate replication, ensuring only 1 round of replication per cell cycle. DnaA can inhibit its own gene expression as well as that of other genes such as dam, rpoH, ftsA and mioC.; Also required for replication of plasmid DNA; binds 4 dnaA boxes in the minimal plasmid RK2 replication origin (oriV).
  • 基因功能参考文献：
    1. analysis of individual DnaA protomers in a complex suggests a mechanism that might support timely assembly of ATP-DnaA on oriC PMID: 29040689
    2. a mechanistic model of datA-IHF.DnaA complex formation and DNA supercoiling-dependent regulation for DDAH. PMID: 27941026
    3. In Escherichia coli, DnaA also shows low residence times in the range of 200 ms and oscillates between spatially opposite chromosome regions in a time frame of one to two seconds, independently of ongoing transcription. PMID: 28166228
    4. Trans-translation ensures timely initiation of DNA replication and DnaA synthesis in Escherichia coli. PMID: 27706629
    5. Cellular acidic phospholipids may positively and negatively influence the initiation activity of DnaA protein to help assure chromosomal replication occurs once, but only once, per cell-cycle. Review. PMID: 23595001
    6. In cells where datA was deleted or an excess of DnaA was provided, cell division occurred in the absence of replication and anucleate cells were formed. PMID: 24574433
    7. The study of mutant DnaAs that are refractory to negative control provide new insight into the mechanisms how DnaA helps regulate replication initiation in Escherichia coli. PMID: 23990329
    8. Data indicate a positive autoregulation of dnaA expression in E. coli. PMID: 23092251
    9. second DnaA-ATP hydrolysis system that occurs at the chromosomal site datA. This locus has been annotated as a reservoir for DnaA that binds many DnaA molecules in a manner dependent upon the nucleoid-associated factor IHF PMID: 23277577
    10. DnaA binds to two oppositely oriented helically phased arrays of low-affinity sites in oriC. PMID: 21895796
    11. Two suppressor mutations in the dnaA gene led to a reduced ability to initiate replication from oriC. PMID: 21299647
    12. A role for nonessential domain II of initiator protein, DnaA, in replication control. PMID: 19546317
    13. DnaA oligomerization at the E. coli replication origin requires the box VII motif of DnaA protein PMID: 15371441
    14. Mutant dnaA is degraded by protein encoded by hspQ.- a novel heat shock protein. PMID: 15569148
    15. residues that might be involved specifically in the cooperative interaction with ATP-DnaA boxes PMID: 15588821
    16. DnaA bound to oriC forms a specific oligomeric structure, which is required to load DnaB helicase PMID: 15878847
    17. the DnaA Arg-285 residue plays a role in the ATP-dependent conformational activation of an initial complex by recognizing ATP bound to DnaA and by modulating the structure of the DnaA multimer to allow interaction with ATP-DnaA-specific binding sites PMID: 15901724
    18. coordinated sequestration of oriC and dnaA is required for maintaining controlled once-per-cell-cycle initiation PMID: 16077105
    19. results suggest that mutant DnaAs with A213D & T435K substitutions suppress the temperature-sensitive phenotype of a dnaX mutant by reducing the frequency of initiation and, therefore, chromosome content per cell PMID: 16225979
    20. two functional states of DnaA exist at high and low membrane occupancy, ascribed to local macromolecular crowding on the membrane surface PMID: 16517983
    21. A new set of rules for identifying efficiently titrating DnaA box regions was formulated and used to analyse sequences for which good titration data are available. PMID: 17316685
    22. These findings suggest a model for DnaA homomultimer formation and DnaB helicase loading on oriC. PMID: 17420252
    23. Results demonstrate that mutant DnaA may be activated at significantly lower concentrations of acidic phospholipids than the wild-type protein. PMID: 17719583
    24. Results show that both DnaA-ATP and DnaA-ADP interacts with Escherichia coli oriC during pre-RC assembly. PMID: 17850252
    25. observations support a model whereby DnaA interacts with the HU alpha dimer or the alphabeta heterodimer, depending on their cellular abundance, to recruit the respective form of HU to oriC PMID: 18179598
    26. ATP-DnaA-oriC unwinding element complexes specifically bound the upper strand of single-stranded DUE PMID: 18216012
    27. Data report that Dps interacts with DnaA and requires the N-terminal region of DnaA to interact. In vitro, Dps inhibits DnaA function in initiation by interfering with strand opening of the replication origin. PMID: 18284581
    28. A GATC cluster inserted at the datA site, which binds large amounts of DnaA in vivo, did not become remethylated at all, unless the availability of the DnaA protein was severely reduced. PMID: 18835566
    29. The 79-135 and 21-27 residues of Domain II of E. coli DnaA are required cell viability. PMID: 18957591
    30. Replication initiation in DnaA-box R4 mutants is insensitive to increased levels of cellular DnaA-ATP. PMID: 19007419
    31. The DnaA protein interacted with RNA polymerase and that it afforded a partial protection from the negative effect of rifampicin. PMID: 19170875
    32. Surplus DnaAcos hyperinitiates DNA Replication and causes lethality at 30degC, 37degC and 42degC, whereas excess DnaA is toxic only at 37degC and 42degC but not at 30degC in a repair-defective strain. PMID: 19432804
    33. Study show that replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle. PMID: 19502409
    34. assignments of 1H, 13C, and 15N resonances of N-terminal domain of Dna A (1-108) which contains the activities of self-oligomerization and DnaB helicase loading PMID: 19636826

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    DnaA family
  • 数据库链接：

    KEGG: ecj:JW3679

    STRING: 316407.85676342