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  4. Recombinant Escherichia coli Arabinose operon regulatory protein (araC)

Recombinant Escherichia coli Arabinose operon regulatory protein (araC)

  • 中文名称：
    Recombinant Escherichia coli Arabinose operon regulatory protein(araC),Yeast
  • 货号：
    CSB-YP364314ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Arabinose operon regulatory protein(araC),Yeast
  • 货号：
    CSB-EP364314ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Arabinose operon regulatory protein(araC),Yeast
  • 货号：
    CSB-EP364314ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Arabinose operon regulatory protein(araC),Yeast
  • 货号：
    CSB-BP364314ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Arabinose operon regulatory protein(araC),Yeast
  • 货号：
    CSB-MP364314ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    araC
  • Uniprot No.：
  • 别名：
    araC; b0064; JW0063; Arabinose operon regulatory protein; HTH-type transcriptional regulator AraC
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-292
  • 氨基酸序列
    MAEAQNDPLL PGYSFNAHLV AGLTPIEANG YLDFFIDRPL GMKGYILNLT IRGQGVVKNQ GREFVCRPGD ILLFPPGEIH HYGRHPEARE WYHQWVYFRP RAYWHEWLNW PSIFANTGFF RPDEAHQPHF SDLFGQIINA GQGEGRYSEL LAINLLEQLL LRRMEAINES LHPPMDNRVR EACQYISDHL ADSNFDIASV AQHVCLSPSR LSHLFRQQLG ISVLSWREDQ RISQAKLLLS TTRMPIATVG RNVGFDDQLY FSRVFKKCTG ASPSEFRAGC EEKVNDVAVK LS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that regulates the expression of several genes involved in the transport and metabolism of L-arabinose. Functions both as a positive and a negative regulator. In the presence of arabinose, activates the expression of the araBAD, araE, araFGH and araJ promoters. In the absence of arabinose, negatively regulates the araBAD operon. Represses its own transcription. Acts by binding directly to DNA.
  • 基因功能参考文献：
    1. Arabinose binding to the AraC dimerization domain changes the properties of the AraC inter-domain linker which connects the dimerization domain to the DNA binding domain via interactions that do not depend on the DNA binding domain. PMID: 26800223
    2. Data [including data from studies of mutant AraC (P8G, P8A)] suggest that, in AraC conformation, interaction of the N-terminal arm with something other than the front lip is the primary determinant of the inducing versus repressing state of AraC. PMID: 25243377
    3. Interaction between domains of AraC in presence of arabinose is reported. Dissociation of arabinose-bound AraC from half-site DNA is faster than free DNA binding domain;affinity of dimeriz'n domains for arabinose is increased when half-site DNA is bound. PMID: 22383259
    4. Mutation in the interdomain affect the dimerization domain-DNA-binding domain interactions in AraC. PMID: 21840981
    5. The allosteric mechanism by which the gene expression regulatory protein AraC regulates its DNA-binding activity is shown to be portable. PMID: 15326589
    6. Apparently, interactions between the N-terminal arm and the dimerization domain core position the arm to interfere with the protein-protein contacts necessary for self-association. PMID: 17132863
    7. The majority of the constitutive mutations lie within the same residues of the N-terminal regulatory arm of AraC. Two, however, were found in the core of the dimerization domain. PMID: 19218388

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ecj:JW0063

    STRING: 316407.21321946