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  4. Recombinant Escherichia coli ATP synthase epsilon chain (atpC)

Recombinant Escherichia coli ATP synthase epsilon chain (atpC)

  • 货号：
    CSB-YP358818ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP358818ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP358818ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP358818ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP358818ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    atpC
  • Uniprot No.：
  • 别名：
    atpC; papG; uncC; b3731; JW3709; ATP synthase epsilon chain; ATP synthase F1 sector epsilon subunit; F-ATPase epsilon subunit
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-139
  • 氨基酸序列
    AMTYHLDVV SAEQQMFSGL VEKIQVTGSE GELGIYPGHA PLLTAIKPGM IRIVKQHGHE EFIYLSGGIL EVQPGNVTVL ADTAIRGQDL DEARAMEAKR KAEEHISSSH GDVDYAQASA ELAKAIAQLR VIELTKKAM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Produces ATP from ADP in the presence of a proton gradient across the membrane.
  • 基因功能参考文献：
    1. Unlike the bovine F1 ATP synthase, an E. coli enzyme contains an epsilon subunit, which is a known inhibitor of bacterial and chloroplast F1 ATPases. (Review) PMID: 26589785
    2. The epsilon-CTD of F1-ATPase has distinct regulatory interactions with F1 when rotary catalysis operates in opposite directions for the hydrolysis or synthesis of ATP. PMID: 26160173
    3. There is a significant predominance of allele papGII above all other alleles or allele combinations. No statistically significant associations could be found between papG allele distribution and the animal breed, organ of isolation and serotype. PMID: 24757808
    4. Vaginal and fecal E.coli isolates from pregnant and nonpregnant women were surveyed for papG alleles by PCR. papG allele II was most prevalent among both vaginal (32.7%) and fecal (3.2%) isolates; other alleles were found only among vaginal isolates. PMID: 23861574
    5. Destabilizing the compact conformation of epsilon's C-terminal domain with a phosphomimetic mutation (epsilonS65D) dramatically increased crystallization success and reproducibility, yielding crystals of E. coli F(1) that diffract to approximately 3.15 A resolution PMID: 23027754
    6. depending on its conformation, epsilon-subunit modulates substrate specificity of TF(o)F(1) PMID: 21454506
    7. Mutants defective in C-terminal regulatory domain exhibited no significant difference in growth rate, molar growth yield, membrane potential, or intracellular ATP concentration under a wide range of growth conditions and stressors. PMID: 21335453
    8. the main function of the C-domain in the Escherichia coli epsilon subunit is to reduce uncoupled ATPase activity, rather than to regulate coupled activity PMID: 16267041

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  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein.
  • 蛋白家族：
    ATPase epsilon chain family
  • 数据库链接：

    KEGG: ecj:JW3709

    STRING: 316385.ECDH10B_3918