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  4. Recombinant Escherichia coli A/G-specific adenine glycosylase (mutY)

Recombinant Escherichia coli A/G-specific adenine glycosylase (mutY)

  • 货号：
    CSB-YP323807ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP323807ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP323807ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP323807ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP323807ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    mutY
  • Uniprot No.：
  • 别名：
    mutY; micA; b2961; JW2928; Adenine DNA glycosylase; EC 3.2.2.31; A/G-specific adenine glycosylase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-350
  • 氨基酸序列
    MQASQFSAQV LDWYDKYGRK TLPWQIDKTP YKVWLSEVML QQTQVATVIP YFERFMARFP TVTDLANAPL DEVLHLWTGL GYYARARNLH KAAQQVATLH GGKFPETFEE VAALPGVGRS TAGAILSLSL GKHFPILDGN VKRVLARCYA VSGWPGKKEV ENKLWSLSEQ VTPAVGVERF NQAMMDLGAM ICTRSKPKCS LCPLQNGCIA AANNSWALYP GKKPKQTLPE RTGYFLLLQH EDEVLLAQRP PSGLWGGLYC FPQFADEESL RQWLAQRQIA ADNLTQLTAF RHTFSHFHLD IVPMWLPVSS FTGCMDEGNA LWYNLAQPPS VGLAAPVERL LQQLRTGAPV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine: 7,8-dihydro-8-oxoguanine (8-oxo-dGTP).
  • 基因功能参考文献：
    1. Studies indicate that the DNA repair gene MUTYH, which is a homologue of the E. coli gene mutY, is responsible for colorectal carcinoma (CRC). PMID: 23933175
    2. MutY mutations reduced the point-mutation rate by 40-60%. PMID: 23248287
    3. ability of MUTYH and the variants to suppress mutations and complement for the absence of MutY in Escherichia coli was assessed using rifampicin resistance assays. PMID: 19836313
    4. catalytic analysis of Escherichia coli MutY DNA glycosylase PMID: 15326180
    5. Mutant MutY(Delta26-134), which lacks the six-helix barrel domain, cannot complement the mutator phenotype of a mutY mutant in vivo. However, the mutant can still bind DNA and has weak catalytic activity at high enzyme concentrations. PMID: 15456766
    6. Electrochemical measurement of MutY using DNA-modified electrodes demonstrates that this base excision repair enzyme containing a [4Fe-4S] cofactor is redox-active when bound to DNA. PMID: 15938629
    7. 8-oxoG-derived lesions are equally miscoding when replicated in E. coli lacking MutY; no significant change in mutation identity or frequency is observed. Yet, in the presence of MutY, Sp1 and Sp2 are more toxic than in cells lacking this repair enzyme PMID: 17260974
    8. Data show a lack of interaction of mutY with dnaX36 since the double dnaX36 mutY strains did not show any major changes in the frequency of G.CT.A transversions compared to the single dnaX36 strain. PMID: 18156258
    9. HPs and SSAs are a common finding in MAP patients. The detection of almost exclusively G:C-->T:A transversions in the K-ras gene of HPs/SSAs strongly suggests that these polyps are related causally to MYH deficiency. PMID: 19013464
    10. V45A and Q182L mutants had reduced binding for A/G and A/8-oxoG mismatches and increased affinities towards T/G mismatch. PMID: 19523222

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  • 蛋白家族：
    Nth/MutY family
  • 数据库链接：

    KEGG: ecj:JW2928

    STRING: 316385.ECDH10B_3136