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  4. Recombinant Escherichia coli 8-oxo-dGTP diphosphatase (mutT)

Recombinant Escherichia coli 8-oxo-dGTP diphosphatase (mutT)

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  • 中文名称：
    大肠杆菌mutT重组蛋白
  • 货号：
    CSB-EP357355ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    mutT
  • Uniprot No.：
  • 别名：
    mutT; b0099; JW0097; 8-oxo-dGTP diphosphatase; 8-oxo-dGTPase; EC 3.6.1.55; 7,8-dihydro-8-oxoguanine-triphosphatase; Mutator protein MutT; dGTP pyrophosphohydrolase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    30.9kDa
  • 表达区域：
    1-129aa
  • 氨基酸序列
    MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGETPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPGEWMSLVGLNADDFPPANEPVIAKLKRL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The Recombinant E.coli K12 mutT protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of mutT gene. This recombinant mutT protein was expressed in the host. The expression region is 1-129aa of the E.coli K12 mutT. In the production, the expression vector contains N-terminal 6xHis-SUMO tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

    The MutT protein in Escherichia coli removes 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-GTP) from the nucleotide pools precluding incorporation into DNA and RNA. 8-oxo-dGTP is a potent mutagenic nucleotide that is readily incorporated into DNA opposite template C or A, with a preference for template A. Therefore, by cleaning the 8-oxo-dGTP pool, MutT reduces spontaneous transversion mutations ∼1000-fold. In addition, MutT can also act on 8-oxo-guanosine triphosphate (8-oxo-GTP) and convert it to 8-oxo-GMP averting mutagenic nucleotide incorporation into RNA. mutT mutants are known to exhibit phenotypes independent of DNA mutational effects, which have been attributed to a decrease in transcription fidelity. The persistence of oxidized ribonucleotides in the available nucleotide pool and the subsequent incorporation of 8-oxo-GTP into mRNA, causing T to G transversions in the nascent transcript, was assumed to be the mechanism for RNA errors and these phenotypes. It was suggested that the absence of MutT can increase the readthrough of a stop codon mutation through 8-oxo-GTP incorporation generating a 30-fold increase in functional protein levels in mutT cultures compared to wild-type cultures. Such RNA infidelity in mutT strains may account for the accumulation of misfolded proteins and the observed cytotoxicity of aminoglycoside antibiotics. mutT phenotypes, such as protein mistranslation or antibiotic sensitivity, that have been attributed to 8-oxo-GTP require other explanations than simply 8-oxo-GTP misincorporation into mRNA.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically hydrolyzes both 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-GTP) to the related monophosphates, thereby cleaning up the nucleotide pools and preventing misincorporation of 8-oxoGua into DNA and RNA. It prevents replicational errors by removing an oxidatively damaged form of guanine (8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP can be inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT may also ensure transcriptional fidelity, removing 8-oxo-GTP from the ribonucleotide triphosphate pool. However, due to the lower efficiency of RNA polymerase 8-oxo-GTP incorporation, MutT is probably not a major contributor to transcriptional fidelity. It also hydrolyzes 8-oxo-dGDP and 8-oxo-GDP to their monophosphate form. In vitro, can also use dGTP, dGDP and other various nucleoside di- and triphosphates, with much lower efficiency. Works cooperatively with MutM and MutY to prevent accumulation in the DNA of oxidized guanine residues.
  • 基因功能参考文献：
    1. The study investigated the role of MutT on transcription fidelity and find no increase in epigenetic switch frequency in the absence of MutT function. PMID: 25294823
    2. MutT mutation increased the point-mutation rate 150-fold. PMID: 23248287
    3. The MutT functions are not needed under anaerobic condition, yet the level of the MutT protein in cell is kept constant, probably for preparing for sudden changes of oxygen pressure. PMID: 21147134
    4. MutT hydrolyzes four types of 8-oxoGua (8-oxo-7,8-dihydroguanine)-containing nucleoside diphosphates and triphosphates to the related monophosphates, thereby preventing misincorporation of 8-oxoGua into DNA and RNA. PMID: 15850400
    5. transient state kinetic analysis of the reaction pathway of mutT; findings confirm an iso kinetic mechanism and explain the preferred hydrolysis of 8-oxo-dGTP PMID: 16285737
    6. These results indicate that mutT plays important roles in mutagenesis induced by 2-OH-dATP and 8-OH-dGTP in vivo. PMID: 17150583
    7. The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications. PMID: 17616589

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  • 蛋白家族：
    Nudix hydrolase family
  • 数据库链接：

    KEGG: ecj:JW0097

    STRING: 316385.ECDH10B_0081