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  4. Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1)

Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1)

  • 中文名称：
    Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1),Yeast
  • 货号：
    CSB-YP315171EEZ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1),Yeast
  • 货号：
    CSB-EP315171EEZ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1),Yeast
  • 货号：
    CSB-EP315171EEZ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1),Yeast
  • 货号：
    CSB-BP315171EEZ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1),Yeast
  • 货号：
    CSB-MP315171EEZ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BMRF1
  • Uniprot No.：
  • 别名：
    BMRF1DNA polymerase processivity factor BMRF1; Early antigen protein D; EA-D; Polymerase accessory subunit
  • 种属：
    Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-404
  • 氨基酸序列
    METTQTLRFK TKALAVLSKC YDHAQTHLKG GVLQVNLLSV NYGGPRLAAV ANAGTAGLIS FEVSPDAVAE WQNHQSPEEA PAAVSFRNLA YGRTCVLGKE LFGSAVEQAS LQFYKRPQGG SRPEFVKLTM EYDDKVSKSH HTCALMPYMP PASDRLRNEQ MIGQVLLMPK TASSLQKWAR QQGSGGVKVT LNPDLYVTTY TSGEACLTLD YKPLSVGPYE AFTGPVAKAQ DVGAVEAHVV CSVAADSLAA ALSLCRIPAV SVPILRFYRS GIIAVVAGLL TSAGDLPLDL SVILFNHASE EAAASTASEP EDKSPRVQPL GTGLQQRPRH TVSPSPSPPP PPRTPTWESP ARPETPSPAI PSHSSNTALE RPLAVQLARK RTSSEARQKQ KHPKKVKQAF NPLI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can you recommend a EBV-/Parvovirus - Peptide/Protein for use in cell culture (T-cell proliferation assay)
Here are the information provided for your reference:
- Searching for EBV-Proteins/Peptides and B19-ProteinsPeptides for use in cell culture (T-cell proliferation assay)
- Name/specific Target: EBV: EA, VCA, EBNA1
- Origin: if possible Protein/Peptide with viral origin. But E.Coli is OK as well
- Conjugation/Tag: if possible not conjugated, but tag is OK as well
- Size: 20 ug

A:
Thanks for your inquiry.
EA: Recombinant Epstein-Barr virus DNA polymerase processivity factor BMRF1 (BMRF1)
CSB-YP315171EEZ >> Yeast .
CSB-EP315171EEZ >> E.coli .
CSB-BP315171EEZ >> Baculovirus .
CSB-MP315171EEZ >> Mammalian cell .
Express region:1-404aa；full length protein.
Tag information:
YP,N terminal 6xHis-tagged；EP,BP,MP : N terminal 10xHis-tagged + C terminal Myc-tagged
Sequence:

METTQTLRFKTKALAVLSKCYDHAQTHLKGGVLQVNLLSVNYGGPRLAAVANAGTAGLISFEV
SPDAVAEWQNHQSPEEAPAAVSFRNLAYGRTCVLGKELFGSAVEQASLQFYKRPQGGSRP
EFVKLTMEYDDKVSKSHHTCALMPYMPPASDRLRNEQMIGQVLLMPKTASSLQKWARQQG
SGGVKVTLNPDLYVTTYTSGEACLTLDYKPLSVGPYEAFTGPVAKAQDVGAVEAHVVCSVA
ADSLAAALSLCRIPAVSVPILRFYRSGIIAVVAGLLTSAGDLPLDLSVILFNHASEEAAASTASE
PEDKSPRVQPLGTGLQQRPRHTVSPSPSPPPPPRTPTWESPARPETPSPAIPSHSSNTALE
RPLAVQLARKRTSSEARQKQKHPKKVKQAFNPLI


Pls refer to:http://www.uniprot.org/uniprot/P0C6Z0

靶点详情

  • 功能：
    Plays an essential role in the viral lytic DNA replication by acting as the polymerase accessory subunit. Stimulates the viral DNA polymerase activity and appears to function with it as a holoenzyme. Increases the processivity of the viral polymerase, probably by acting as a sliding clamp that prevents dissociation of the polymerase from the active template. In addition, BMRF1 transcriptionally activates the early BHLF1 promoter.
  • 基因功能参考文献：
    1. BMRF1 is essential for EBV lytic protein synthesis and lytic DNA replication. PMID: 16641300
  • 亚细胞定位：
    Virion tegument. Host nucleus.
  • 蛋白家族：
    Herpesviridae DNA polymerase accessory subunit family
  • 数据库链接：

    KEGG: vg:3783718