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  4. Recombinant Drosophila melanogaster Ovarian-specific serine/threonine-protein kinase Lok (lok)

Recombinant Drosophila melanogaster Ovarian-specific serine/threonine-protein kinase Lok (lok)

  • 中文名称：
    黑腹果蝇lok重组蛋白
  • 货号：
    CSB-YP523992DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇lok重组蛋白
  • 货号：
    CSB-EP523992DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇lok重组蛋白
  • 货号：
    CSB-EP523992DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇lok重组蛋白
  • 货号：
    CSB-BP523992DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇lok重组蛋白
  • 货号：
    CSB-MP523992DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lok
  • Uniprot No.：
  • 别名：
    dMNK; lok; LOK_DROME; Ovarian-specific serine/threonine-protein kinase Lok; Protein loki
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-476
  • 氨基酸序列
    MARDTQGTQG TQSQASNIWT QVESQPMEKI VWGRLYGKNI KIKSLGTSSK YRIIYTHSSF SVDLNNDEFT AGRGEANDLI LTLNDLPEKI LTRISKVHFI IKRANCELTN PVYIQDLSRN GTFVNNEKIG TNRMRILKND DVISLSHPTY KAFVFKDLSP NESIGLPEEI NKTYYVNRKL GSGAYGLVRL VYDTRTCQQF AMKIVKKNML SGARPSTNFS DPDRVLNEAK IMKNLSHPCV VRMHDIVDKP DSVYMVLEFM RGGDLLNRII SNKLLSEDIS KLYFYQMCHA VKYLHDRGIT HRDLKPDNVL LETNDEETLL KVSDFGLSKF VQKDSVMRTL CGTPLYVAPE VLITGGREAY TKKVDIWSLG VVLFTCLSGT LPFSDEYGTP AAQQIKKGRF AYGHPSWKSV SQRAKLLINQ MLIVDPERRP SIDDVLQSSW LRDAPMLQKA KRLMKLDGME IEEENFLEPP TKRSRR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May have a role in germline establishment.
  • 基因功能参考文献：
    1. this study demonstrates, that Lok kinase activation is required for the DNA damage-mediated disruption of adult stem cell self-renewal and lineage differentiation, and might also offer novel insight into how DNA damage causes tissue aging and cancer formation. PMID: 27729408
    2. FHA domain in Mnk plays dual functions in mediating embryonic DNA damage responses by means of its phosphopeptide-binding ability: activating Mnk in nucleus upon DNA damage &recruiting Mnk to multiple subcellular structures independently of DNA damage PMID: 25808488
    3. We conclude that the primary responsibility for maintaining genome integrity in the male germline lies with Chk2, and that P53 is required to reconstitute the germline when cells are eliminated owing to unrepaired DNA damage. PMID: 24586185
    4. nuclear retention involves Chk2-mediated inactivation of the Drosophila stem loop binding protein (SLBP), the levels of which are specifically depleted in damaged nuclei following Chk2 phosphorylation, an event that contributes to nuclear fallout. PMID: 24835465
    5. while Chk2 functions by activating the p53-dependent apoptotic cascade, Chk2 also functions independently of p53 to limit survival. PMID: 21655087
    6. Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. PMID: 20838898
    7. The DNA damage-activated kinases Chk1 and Chk2 may be involved in tau phosphorylation and toxicity in the pathogenesis of Alzheimer's disease. PMID: 20159774
    8. plays a crucial role in the DNA damage response pathway mediating cell cycle arrest and apoptosis, and the ATM-Chk2 pathway is likely conserved in Drosophila PMID: 11728459
    9. regulates irradiation-induced, p53-mediated apoptosis in Drosophila PMID: 12172011
    10. in flies, chk2 is involved in monitoring double-strand breaks (DSBs) caused by irradiation during S and G2 phases PMID: 12663536
    11. Dmnk plays an important role in a mitotic checkpoint in syncytial embryos. PMID: 12804777
    12. Drosophila has Chk2- and p53-independent pathways to activate caspases and induce apoptosis in response to IR. PMID: 16785441
    13. The mnk (also known as loki) gene encodes Checkpoint kinase 2 (Chk2), which functions in DNA-damage signal transduction. PMID: 17409117
    14. Genetic screening was performed to identify novel components of the Chk2 signaling pathway in Drosophila. PMID: 18612247
    15. Data propose that hid expression is activated in a pole cell-specific manner by loki/p53 and dpp/egr during embryogenesis. PMID: 19382940

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  • 亚细胞定位：
    Nucleus speckle. Note=Speckled subnuclear compartment.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CDS1 subfamily
  • 组织特异性：
    In stage 3 embryos, both isoforms are expressed in both somatic and pole cell nuclei. Expression in pole cell nuclei is sustained until stage 9 and weakly expressed after pole cell invagination into the abdominal cavity.
  • 数据库链接：

    KEGG: dme:Dmel_CG10895

    STRING: 7227.FBpp0080860

    UniGene: Dm.475