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  4. Recombinant Drosophila melanogaster Negative elongation factor E (Nelf-E)

Recombinant Drosophila melanogaster Negative elongation factor E (Nelf-E)

  • 中文名称：
    黑腹果蝇Nelf-E重组蛋白
  • 货号：
    CSB-YP019521DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Nelf-E重组蛋白
  • 货号：
    CSB-EP019521DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Nelf-E重组蛋白
  • 货号：
    CSB-EP019521DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Nelf-E重组蛋白
  • 货号：
    CSB-BP019521DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Nelf-E重组蛋白
  • 货号：
    CSB-MP019521DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nelf-E
  • Uniprot No.：
  • 别名：
    Nelf-E; anon-66Da; CG5994; Negative elongation factor E
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-280
  • 氨基酸序列
    MVYIHFPNNL TEEEQMLQAK YQKLKKKKKA LQAHKAPKPE PESSLTLKRP TDARDAREVA RKLIKSGAIP AIQKQTKQDQ TSFKRPKGQE RAKRSTSETT VASYQPFSST QNDVAQETII SEIIKEEPRR QNLYQHFATE RDREERGMPE KVPMDTAQPE KPRAGNTIFV SGNKVTEDFL KKTFNDYGTI VNVSMEIEKS RGFVSFAKPE SADRAIAEIH GKNVNGINLQ VQLARRQPQI EPINDASSSA VWSSIAASKS QKGSHKDHRE MVQYDEDFLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex, causes transcriptional pausing.
  • 基因功能参考文献：
    1. biochemical analysis reveals that the sequence-specific transcription factor, GAF, orchestrates efficient pausing by recruiting NELF to promoters before transcription initiation and by assisting in loading NELF onto Pol II after initiation PMID: 23746353
    2. The finding that both phenotypes of arrested embryos are obtained in embryos that lack maternally provided NELF-A as well as in embryos with reduced levels of maternal NELF-E show that these phenotypes result from the reduced activity of the NELF complex. PMID: 20634899
    3. propose that NELF and DSIF cause polymerase to pause in the promoter proximal region of hsp70 PMID: 12782658
  • 亚细胞定位：
    Nucleus. Chromosome. Note=Associates with polytene chromosomes. Associates with the hsp70 promoter when it is inactive, but not when it is activated. The NELF complex possibly dissociates from chromatin following phosphorylation of RNA polymerase II.
  • 蛋白家族：
    RRM NELF-E family
  • 数据库链接：

    KEGG: dme:Dmel_CG5994

    STRING: 7227.FBpp0076374

    UniGene: Dm.20046