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  4. Recombinant Drosophila melanogaster Myeloid leukemia factor (Mlf)

Recombinant Drosophila melanogaster Myeloid leukemia factor (Mlf)

  • 中文名称：
    黑腹果蝇Mlf重组蛋白
  • 货号：
    CSB-YP882055DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Mlf重组蛋白
  • 货号：
    CSB-EP882055DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Mlf重组蛋白
  • 货号：
    CSB-EP882055DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Mlf重组蛋白
  • 货号：
    CSB-BP882055DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Mlf重组蛋白
  • 货号：
    CSB-MP882055DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mlf
  • Uniprot No.：
  • 别名：
    Mlf; CG8295; Myeloid leukemia factor; Myelodysplasia-myeloid leukemia factor; dMLF
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-376
  • 氨基酸序列
    MSLFGALMGD FDDDLGLMNN HMNHTMNAMN MQMRSMNRLM NSFMPDPFMQ VSPFDQGFQQ NALMERPQMP AMPAMGLFGM PMMPNFNRLL NADIGGNSGA SFCQSTVMTM SSGPDGRPQI YQASTSTKTG PGGVRETRRT VQDSRTGVKK MAIGHHIGER AHIIEKEQDM RSGQLEERQE FINLEEGEAE QFDREFTSRA SRGAVQSRHH AGGMQAIMPA RPAAHTSTLT IEPVEDDDDD DDDCVIQEQQ PVRSSAGRHY SSAPTAPQNS GNIATAAAPT PTSSPTTYDT SNGNNNNYVS SRRSYLRNGH GHSLATPRRP LRTPPSSPLA TVSTSPSIHP HPYAANPRRQ QRAVKHFHTE DEAASSYKAV KRGKKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. Mlf and its mutant were characterized. Mlf can act developmentaly and post-developmentally to suppress neurodegeneration and premature aging in a cerebellar ataxia model. PMID: 17121541
    2. MLF1 has been described as mainly cytoplasmic, but in Drosophila, one of the dMLF isoforms (dMLFA) localized mainly in the nucleus. PMID: 18159124
    3. Data revealed that MLF interacts with CSN3 in vivo and that the PCI domain of the dCSN3 protein is sufficient for this to occur, possibly functioning as a structural scaffold for assembly of the COP9 signalosome complex. PMID: 18199288
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    MLF family
  • 组织特异性：
    Expressed at high levels in unfertilized eggs, early embryos, pupae and adult males while a low level expression is found in adult females and larvae.
  • 数据库链接：

    KEGG: dme:Dmel_CG8295

    STRING: 7227.FBpp0089346

    UniGene: Dm.12915