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  4. Recombinant Drosophila melanogaster GTP-binding nuclear protein Ran (ran)

Recombinant Drosophila melanogaster GTP-binding nuclear protein Ran (ran)

  • 中文名称：
    黑腹果蝇Ran重组蛋白
  • 货号：
    CSB-YP894758DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Ran重组蛋白
  • 货号：
    CSB-EP894758DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Ran重组蛋白
  • 货号：
    CSB-EP894758DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Ran重组蛋白
  • 货号：
    CSB-BP894758DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Ran重组蛋白
  • 货号：
    CSB-MP894758DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ran
  • Uniprot No.：
  • 别名：
    Ran; Ran10A; CG1404; GTP-binding nuclear protein Ran; Ras-related nuclear protein
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-216
  • 氨基酸序列
    MAQEGQDIPT FKCVLVGDGG TGKTTFVKRH MTGEFEKKYV ATLGVEVHPL IFHTNRGAIR FNVWDTAGQE KFGGLRDGYY IQGQCAVIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLVG DPNLEFVAMP ALLPPEVKMD KDWQAQIERD LQEAQATALP DEDEEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. GTP-bound Ran modulates both spindle and nuclear envelope assembly, supporting a role during mitosis. During oogenesis, modulates formation of Nup358-containing granules and biogenesis of the nuclear pore complex probably by mediating the transport of their components along microtubules.
  • 基因功能参考文献：
    1. Results demonstrate that Ran is associated with the resistance to and tolerance of multiple pesticides. PMID: 29680247
    2. RanGTP is required for meiotic spindle organization and the initiation of embryonic development. PMID: 22100918
    3. the Canoe Ras-association (RA) domains directly bind RanGTP PMID: 22024168
    4. Knockdown of the small G protein Ran, which is essential for nuclear transport, leads to an arrest of EcR in the cytoplasm, but does not prevent efficient nuclear import of the most important heterodimerization partner of EcR, ultraspiracle (Usp). PMID: 21125558
    5. gtpase ran localizes around the microtubule spindle in vivo during mitosis in Drosophila embryos PMID: 12121620

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  • 亚细胞定位：
    Nucleus. Nucleus envelope. Cytoplasm. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Small GTPase superfamily, Ran family
  • 数据库链接：

    KEGG: dme:Dmel_CG1404

    STRING: 7227.FBpp0073328

    UniGene: Dm.6755