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  4. Recombinant Drosophila melanogaster Discs overgrown protein kinase (dco)

Recombinant Drosophila melanogaster Discs overgrown protein kinase (dco)

  • 中文名称：
    Recombinant Drosophila melanogaster Discs overgrown protein kinase(dco),Yeast
  • 货号：
    CSB-YP527991DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Drosophila melanogaster Discs overgrown protein kinase(dco),Yeast
  • 货号：
    CSB-EP527991DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Drosophila melanogaster Discs overgrown protein kinase(dco),Yeast
  • 货号：
    CSB-EP527991DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Drosophila melanogaster Discs overgrown protein kinase(dco),Yeast
  • 货号：
    CSB-BP527991DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Drosophila melanogaster Discs overgrown protein kinase(dco),Yeast
  • 货号：
    CSB-MP527991DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    dco
  • Uniprot No.：
  • 别名：
    dco; dbt; CG2048; Discs overgrown protein kinase; EC 2.7.11.1; Protein double-time
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-440
  • 氨基酸序列
    MELRVGNKYR LGRKIGSGSF GDIYLGTTIN TGEEVAIKLE CIRTKHPQLH IESKFYKTMQ GGIGIPRIIW CGSEGDYNVM VMELLGPSLE DLFNFCSRRF SLKTVLLLAD QMISRIDYIH SRDFIHRDIK PDNFLMGLGK KGNLVYIIDF GLAKKFRDAR SLKHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG YVLMYFNLGA LPWQGLKAAN KRQKYERISE KKLSTSIVVL CKGFPSEFVN YLNFCRQMHF DQRPDYCHLR KLFRNLFHRL GFTYDYVFDW NLLKFGGPRN PQAIQQAQDG ADGQAGHDAV AAAAAVAAAA AASSHQQQQH KVNAALGGGG GSAAQQQLQG GQTLAMLGGN GGGNGSQLIG GNGLNMDDSM AATNSSRPPY DTPERRPSIR MRQGGGGGGG GVGVGGMPSG GGGGGVGNAK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in circadian rhythms, viability and molecular oscillations of the clock genes period (per) and timeless (tim). Dbt reduces the stability and thus the accumulation of monomeric per proteins, probably through phosphorylation. No evident circadian oscillation is detected in head. Together with CkIalpha, regulates processing of ci by phosphorylating it which promotes its binding to slmb, the F-box recognition component of the SCF(slmb) E3 ubiquitin-protein ligase.
  • 基因功能参考文献：
    1. we show that spag knock-down or reduced Dbt activity in circadian cells leads to longer circadian periods and expression of activated initiator caspase only during the middle of the day. PMID: 25951229
    2. Responses of the clock to light via the regulated translation of DBT. PMID: 25211129
    3. Data show that the HSP-90 cochaperone spaghetti protein (SPAG) antagonizes casein kinase I epsilon DBT autophosphorylation in S2 cells PMID: 25939385
    4. Data demonstrate that overall reduction of DBT causes long and arrhythmic behavior revealing an unexpected role in promoting synchrony of the circadian clock network. PMID: 24820422
    5. The introduction of CKI-epsilon point mutation L39Q, found in human breast cancer, into the fly homolog dco causes an overgrowth phenotype in imaginal disc tissue. Adding S101R, modifies the mutant phenotype so that the affected tissue disintegrates. PMID: 20979026
    6. expression of DBT in Drosophila S2 cells produced a CKI-7-sensitive kinase activity which was reduced by both the dbt(S) and dbt(L) mutations of circadian rhythm PMID: 14701759
    7. Dco coordinates tissue size by stimulating cell division/growth and blocking apoptosis via activation of DIAP1 expression. PMID: 17134692
    8. This first analysis of adult flies with a virtual lack of DBT activity demonstrates that DBT's kinase activity is necessary for normal circadian rhythms and that a general reduction of DBT kinase activity does not produce short periods. PMID: 17893330
    9. human casein kinase 1 epsilon cannot replace the activity of dbt in flies despite the high degree of similarity in primary sequence and kinase function PMID: 18258753
    10. heterozygous mutations of this gene, which encodes the major catalytic subunit of PKA (cAMP-dependent kinase), delayed AMI onset by more than 2-fold without affecting lifespan and memory at young age.(review) PMID: 18646611
    11. The results demonstrate a high degree of evolutionary conservation of fly and vertebrate CKIdelta and of the functions affected by their period-shortening mutations. PMID: 18957703
    12. These results suggest that DBT regulates phosphorylation, stability and localization of PDP1epsilon, and that it has multiple targets in the Drosophila circadian system. PMID: 19663814

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  • 蛋白家族：
    Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
  • 组织特异性：
    Expressed in photoreceptor cells of the eyes as well as in the region situated between the optic lobe and the central brain.
  • 数据库链接：

    KEGG: dme:Dmel_CG2048

    STRING: 7227.FBpp0085104

    UniGene: Dm.1901