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  4. Recombinant Dog Methylosome subunit pICln (CLNS1A)

Recombinant Dog Methylosome subunit pICln (CLNS1A)

  • 中文名称：
    Recombinant Dog Methylosome subunit pICln(CLNS1A),Yeast
  • 货号：
    CSB-YP005572DO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Dog Methylosome subunit pICln(CLNS1A),Yeast
  • 货号：
    CSB-EP005572DO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Dog Methylosome subunit pICln(CLNS1A),Yeast
  • 货号：
    CSB-EP005572DO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Dog Methylosome subunit pICln(CLNS1A),Yeast
  • 货号：
    CSB-BP005572DO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Dog Methylosome subunit pICln(CLNS1A),Yeast
  • 货号：
    CSB-MP005572DO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CLNS1A
  • Uniprot No.：
  • 别名：
    CLNS1A; ICLNMethylosome subunit pICln; Chloride channel; nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; I(Cln)
  • 种属：
    Canis familiaris (Dog) (Canis lupus familiaris)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-235
  • 氨基酸序列
    SFLKSFPPP GSAEGLRQQQ PETEAVLNGK GLGTGTLYIA ESRLSWLDGS GLGFSLEYPT ISLHAVSRDL NAYPREHLYV MVNAKFGEES KESVAEEEDS DDDVEPIAEF RFVPSDKSAL EAMFTAMCEC QALHPDPEDE DSDDYDGEEY DVEAHEQGQG DIPTFYTYEE GLSHLTAEGQ ATLERLEGML SQSVSSQYNM AGVRTEDSTR DYEDGMEVDT TPTVAGQFED ADVDH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
  • 基因功能参考文献：
    1. The C-terminus of ICln adopts a preferred alpha-helical organization between residues E170 and E187, and exists preferentially in extended conformations (beta-strands) between residues D161 to Y168 and E217 to T223. PMID: 22179008
    2. Data show that ICln159, a truncated ICln mutant, belongs to the pleckstrin homology (PH) domain family of proteins and interacts with LSm4, a protein involved in splicing and mRNA degradation. PMID: 15905169
  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    PICln (TC 1.A.47) family
  • 数据库链接：

    KEGG: cfa:403971

    STRING: 9615.ENSCAFP00000007306

    UniGene: Cfa.3840