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Recombinant Dog Inactive pancreatic lipase-related protein 1 (PNLIPRP1)

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  • 中文名称：
    Recombinant Dog Inactive pancreatic lipase-related protein 1(PNLIPRP1)
  • 货号：
    CSB-YP018263DO
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP018263DO could indicate that this peptide derived from Yeast-expressed Canis lupus familiaris (Dog) (Canis familiaris) PNLIPRP1.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP018263DO could indicate that this peptide derived from Yeast-expressed Canis lupus familiaris (Dog) (Canis familiaris) PNLIPRP1.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    PNLIPRP1
  • Uniprot No.：
  • 别名：
    PNLIPRP1; PLRP1; Inactive pancreatic lipase-related protein 1; PL-RP1
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    51.7 kDa
  • 表达区域：
    18-467aa
  • 氨基酸序列
    KEVCYEQIGCFSDAEPWAGTAIRPLKVLPWSPERIGTRFLLYTNKNPNNFQTLLPSDPSTIEASNFQTDKKTRFIIHGFIDKGEENWLLDMCKNMFKVEEVNCICVDWKKGSQTSYTQAANNVRVVGAQVAQMLSMLSANYSYSPSQVQLIGHSLGAHVAGEAGSRTPGLGRITGLDPVEASFQGTPEEVRLDPTDADFVDVIHTDAAPLIPFLGFGTSQQMGHLDFFPNGGEEMPGCKKNALSQIVDLDGIWEGTRDFVACNHLRSYKYYSESILNPDGFASYPCASYRAFESNKCFPCPDQGCPQMGHYADKFAVKTSDETQKYFLNTGDSSNFARWRYGVSITLSGKRATGQAKVALFGSKGNTHQFNIFKGILKPGSTHSNEFDAKLDVGTIEKVKFLWNNNVVNPTFPKVGAAKITVQKGEEKTVHSFCSESTVREDVLLTLTPC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I bought cPL recombinant protein Recombinant Dog Inactive pancreatic lipase-related protein 1(PNLIPRP1) N-terminal 6xHis-tagged (03613) CSB-YP018263DO (order M1-69098)
I tried to test it and diluted the protein 500ug by 1ml water but the powder is not diluted absolutely.
I did vortexing to protein and waited for a while.
The pallet is not dissolved until now. Could you tell me the reason?

A:
Thanks for your inquiry. Our tech team confirms that we test the reconstitution before shipping. This lyophilized protein could be dissolved in deionized sterile water well.
The isoelectric point(pI) of this protein is 6.1, it’s a acidic protein. Our tech team suggest you to add 0.1M NaHCO3 to help resuspension. Dissolving the protein into sterile water, and then adding 0.1M NaHCO3 drop by drop. Stir gently and dissolve at room temperature 30 min. If it still does not work, pls send the picture of the protein after dissolution for us to analyze.
Here is information about reconstitution for your reference.
Reconstitution
The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on your experiment demand.)
It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration. If you have special requirements for concentration, then you could dilute the protein according to your experiment demand.
We haven’t studied whether the optimal concentration range will vary among different hosts such as e. coli, yeast, mammalian or baculovirus, so we can’t provide usable advice.
If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage. We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on your experiment demand.
In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.

靶点详情

  • 功能：
    May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro).
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Detected in pancreas (at protein level).
  • 数据库链接：

    KEGG: cfa:404010

    STRING: 9615.ENSCAFP00000017453

    UniGene: Cfa.3881