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  4. Recombinant Clostridium perfringens Perfringolysin O (pfo)

Recombinant Clostridium perfringens Perfringolysin O (pfo)

  • 中文名称：
    产气荚膜梭状芽胞杆菌pfo重组蛋白
  • 货号：
    CSB-YP611683CAAN
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    产气荚膜梭状芽胞杆菌pfo重组蛋白
  • 货号：
    CSB-EP611683CAAN
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    产气荚膜梭状芽胞杆菌pfo重组蛋白
  • 货号：
    CSB-EP611683CAAN-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    产气荚膜梭状芽胞杆菌pfo重组蛋白
  • 货号：
    CSB-BP611683CAAN
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    产气荚膜梭状芽胞杆菌pfo重组蛋白
  • 货号：
    CSB-MP611683CAAN
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    pfo; pfoA; pfoR; CPF_0156; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin
  • 种属：
    Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-500
  • 氨基酸序列
    KD ITDKNQSIDS GISSLSYNRN EVLASNGDKI ESFVPKEGKK AGNKFIVVER QKRSLTTSPV DISIIDSVND RTYPGALQLA DKAFVENRPT ILMVKRKPIN INIDLPGLKG ENSIKVDDPT YGKVSGAIDE LVSKWNEKYS STHTLPARTQ YSESMVYSKS QISSALNVNA KVLENSLGVD FNAVANNEKK VMILAYKQIF YTVSADLPKN PSDLFDDSVT FNDLKQKGVS NEAPPLMVSN VAYGRTIYVK LETTSSSKDV QAAFKALIKN TDIKNSQQYK DIYENSSFTA VVLGGDAQEH NKVVTKDFDE IRKVIKDNAT FSTKNPAYPI SYTSVFLKDN SVAAVHNKTD YIETTSTEYS KGKINLDHSG AYVAQFEVAW DEVSYDKEGN EVLTHKTWDG NYQDKTAHYS TVIPLEANAR NIRIKARECT GLAWEWWRDV ISEYDVPLTN NINVSIWGTT LYPGSSITYN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
  • 基因功能参考文献：
    1. R468A mutation in perfringolysin O destabilizes toxin structure and induces membrane fusion. PMID: 28263714
  • 亚细胞定位：
    Secreted. Host cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Thiol-activated cytolysin family
  • 数据库链接：

    KEGG: cpf:CPF_0156

    STRING: 195103.CPF_0156