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  4. Recombinant Caulobacter crescentus Chromosomal replication initiator protein DnaA (dnaA)

Recombinant Caulobacter crescentus Chromosomal replication initiator protein DnaA (dnaA)

  • 中文名称：
    克氏结肠杆菌dnaA重组蛋白
  • 货号：
    CSB-YP488758DQP
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    克氏结肠杆菌dnaA重组蛋白
  • 货号：
    CSB-EP488758DQP
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    克氏结肠杆菌dnaA重组蛋白
  • 货号：
    CSB-EP488758DQP-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    克氏结肠杆菌dnaA重组蛋白
  • 货号：
    CSB-BP488758DQP
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    克氏结肠杆菌dnaA重组蛋白
  • 货号：
    CSB-MP488758DQP
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    dnaA
  • Uniprot No.：
  • 别名：
    dnaA; CCNA_00008Chromosomal replication initiator protein DnaA
  • 种属：
    Caulobacter crescentus (strain NA1000 / CB15N)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-490
  • 氨基酸序列
    MTMKGGVASQ DFSAAIATAC EPAANVWSKV CVALKRELGD AAFGSWIAPA MLREAATGDV VLVTSTGIAR DWIRRSAWRR IGELWAAHDA TGRRIDLKSR LEFEAAAGAY VEATPKAVAA EPIEIVLPVS TDAPTVVAPS AKSPRTQGLQ ERFTFETFVP GPANEFAHAV ARRIANWADG HFNPVLFHGP YGFGKTHLLN ALAWEAMRNA PEKRVVYLTA ERFLSTFVRA VMDRQTAAFK EELRAADLLI IDDVHFIAGK QSTQEELFHT LTALVGEGGR VVFSADRPPS AMTEMDAHLR SHLSAGLVCG LEPADRNLRL GILERKIQTL GAAHGFEPSI RPEVMQFLAD RFTDSVRELE GALNTLSARA GEGLSRMTLD EVQAILRPHL RSGEKRITID DIQKATAEHY GMKQADLLSE RRNRAVARPR QAAMWLAKQL TTRSLPDIGR RFGGRDHTTV LHAVRRIEAL RAEDSALSHD LETLTRKLRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
  • 基因功能参考文献：
    1. The AAA+ domain of DnaA may play a role in temporally regulating the bifunctionality of DnaA by reallocating DnaA molecules from initiating DNA replication to transcribing genes within the unique DnaA regulon of C. crescentus. PMID: 22022497
    2. DnaA binds G-boxes with moderate and W-boxes with very weak affinities. PMID: 21843309
    3. The results suggest that the trans translation capacity of the cell is sensed through the dnaA promoter to control the timing of DNA replication initiation PMID: 19429626
    4. The transcription of HdaA is directly activated by DnaA, providing a robust feedback regulatory mechanism that adjusts the levels of HdaA to inactivate DnaA. PMID: 19633089
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    DnaA family
  • 数据库链接：

    KEGG: ccs:CCNA_00008