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  4. Recombinant Canary circovirus Replication-associated protein (Rep)

Recombinant Canary circovirus Replication-associated protein (Rep)

  • 中文名称：
    Recombinant Canary circovirus Replication-associated protein(Rep),Yeast
  • 货号：
    CSB-YP856226CLAU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Canary circovirus Replication-associated protein(Rep),Yeast
  • 货号：
    CSB-EP856226CLAU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Canary circovirus Replication-associated protein(Rep),Yeast
  • 货号：
    CSB-EP856226CLAU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Canary circovirus Replication-associated protein(Rep),Yeast
  • 货号：
    CSB-BP856226CLAU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Canary circovirus Replication-associated protein(Rep),Yeast
  • 货号：
    CSB-MP856226CLAU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rep
  • Uniprot No.：
  • 别名：
    Rep; ORF1; Replication-associated protein; EC 2.7.7.-; EC 3.1.21.-; EC 3.6.1.3; ATP-dependent helicase Rep; RepP
  • 种属：
    Canary circovirus (CaCV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-290
  • 氨基酸序列
    MAPVRAAAAK RWCFTLNNYT AEEEAKVRAL LPGEFHFAIC GKERGEQGTP HLQGFLHFKK KQRLSALKKL LARAHWEKAR GSDHDNEEYC SKENDVILTI GEPVQGNRSD LAGAVAAVKA GRRMVDIARE FSEIYVKYGR GLRDLALMIG QKPRDFKTEV VVITGPSGVG KSRLASEMEG SKFYKMKGDW WDGYSNEDIV IMDDFYGWLP FCEMLRLMDR YPHKVPVKGS YVEFTSKKIV ITSNTHPESW YCPDKCYLPA LFRRINKWMY WDGLRFEDVP DAMKKHPINY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep.
  • 亚细胞定位：
    Host nucleus.
  • 蛋白家族：
    Nanoviruses/circoviruses replication-associated protein family
  • 数据库链接：

    KEGG: vg:935131