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  4. Recombinant Burkholderia xenovorans 4-hydroxy-2-oxovalerate aldolase 4 (bphI)

Recombinant Burkholderia xenovorans 4-hydroxy-2-oxovalerate aldolase 4 (bphI)

  • 货号：
    CSB-YP344308BNV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP344308BNV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP344308BNV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP344308BNV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP344308BNV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    bphI
  • Uniprot No.：
  • 别名：
    bphI; Bxeno_C1121; Bxe_C11874-hydroxy-2-oxovalerate aldolase 4; HOA 4; EC 4.1.3.39; 4-hydroxy-2-keto-pentanoic acid aldolase 4; 4-hydroxy-2-oxohexanoate aldolase; EC 4.1.3.43; 4-hydroxy-2-oxopentanoate aldolase 4
  • 种属：
    Burkholderia xenovorans (strain LB400)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-346
  • 氨基酸序列
    MKLEGKKVTV HDMTLRDGMH PKRHQMTLEQ MKSIACGLDA AGIPLIEVTH GDGLGGSSVN YGFPAHSDEE YLGAVIPLMK QAKVSALLLP GIGTVEHLKM AKDLGVNTIR VATHCTEADV SEQHITQSRK LGLDTVGFLM MAHMASPEKL VSQALLMQGY GANCIYVTDS AGYMLPDDVK ARLSAVRAAL KPETELGFHG HHNLAMGVAN SIAAIEAGAT RIDAAAAGLG AGAGNTPMEV FIAVCARMGI ETGVDVFKIQ DVAEDLVVPI MDHVIRIDRD SLTLGYAGVY SSFLLFAKRA SAKYGVPARD ILVELGRRGM VGGQEDMIED TAMTMARERG LTLTAA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the retro-aldol cleavage of both 4-hydroxy-2-oxopentanoate (HOPA) and 4-hydroxy-2-oxohexanoate (HOHA) to pyruvate and acetaldehyde or propanaldehyde, respectively. The aldehydes produced by this reaction are directly channeled from BphI to the dehydrogenase BphJ, ensuring that these toxic aldehydes are sequestered from cellular components. Is involved in the meta-cleavage pathway for the degradation of polychlorinated biphenyls (PCBs). Appears to be stereospecific since it can cleave (4S)-4-hydroxy-2-oxopentanoate but not the (4R) isomer. Also exhibits a secondary oxaloacetate decarboxylase activity. Finally, is also able to catalyze the reverse reaction, albeit much less efficiently, i.e. the condensation of aldehyde acceptors of two to three carbons in length with pyruvate. This aldol addition reaction is stereospecific; the condensation of acetaldehyde and pyruvate with BphI produces only the (4S)-4-hydroxy-2-oxopentanoate isomer. Aldehyde channeling in the BphI-BphJ complex can occur in reverse, from the dehydrogenase to the aldolase active sites, and the BphJ reductive deacylation reaction increases 4-fold when BphI is catalyzing the aldol addition reaction. Therefore, the BphI-BphJ enzyme complex exhibits unique bidirectionality in substrate channeling and allosteric activation.
  • 基因功能参考文献：
    1. Levels of activation of bphI by bphJ N170A, N170D, and I171A were reduced by >/=3-fold in the presence of NADH and >/=4.5-fold when bphJ was undergoing turnover, indicating that allosteric activation of the aldolase has been compromised in these variants. PMID: 22574886
  • 蛋白家族：
    4-hydroxy-2-oxovalerate aldolase family
  • 数据库链接：

    KEGG: bxb:DR64_8618

    STRING: 266265.Bxe_C1187