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  4. Recombinant Burkholderia pseudomallei Translocator protein BipD (bipD)

Recombinant Burkholderia pseudomallei Translocator protein BipD (bipD)

  • 中文名称：
    Recombinant Burkholderia pseudomallei Translocator protein BipD(bipD),Yeast
  • 货号：
    CSB-YP734020BXW
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Burkholderia pseudomallei Translocator protein BipD(bipD),Yeast
  • 货号：
    CSB-EP734020BXW
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Burkholderia pseudomallei Translocator protein BipD(bipD),Yeast
  • 货号：
    CSB-EP734020BXW-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Burkholderia pseudomallei Translocator protein BipD(bipD),Yeast
  • 货号：
    CSB-BP734020BXW
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Burkholderia pseudomallei Translocator protein BipD(bipD),Yeast
  • 货号：
    CSB-MP734020BXW
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    bipD
  • Uniprot No.：
  • 别名：
    bipD; BPSS1529Translocator protein BipD
  • 种属：
    Burkholderia pseudomallei (strain K96243)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-310
  • 氨基酸序列
    MNMHVDMGRA LTVRDWPALE ALAKTMPADA GARAMTDDDL RAAGVDRRVP EQKLGAAIDE FASLRLPDRI DGRFVDGRRA NLTVFDDARV AVRGHARAQR NLLERLETEL LGGTLDTAGD EGGIQPDPIL QGLVDVIGQG KSDIDAYATI VEGLTKYFQS VADVMSKLQD YISAKDDKNM KIDGGKIKAL IQQVIDHLPT MQLPKGADIA RWRKELGDAV SISDSGVVTI NPDKLIKMRD SLPPDGTVWD TARYQAWNTA FSGQKDNIQN DVQTLVEKYS HQNSNFDNLV KVLSGAISTL TDTAKSYLQI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for invasion of epithelial cells, as well as for survival within host cells, escape from endocytic vesicles and subsequent actin-tail formation. Probably regulates the secretion of effectors BipB and BipC and their final integration into the target cell membrane.
  • 基因功能参考文献：
    1. BipD has been crystallized in a monoclinic crystal form that diffracts X-rays to 2.5 angstroms resolution PMID: 16880550
    2. Residues 10-310 of mature BipD, a component of B. pseudomallei type III secretion system, has been crystallized. Native BipD crystals have undergone preliminary crystallographic analysis. PMID: 16946464
    3. The crystal structure og BipD suggests that the biologically active form of the molecule may be a dimer formed by contacts involving the C-terminal alpha-helix, which is the most strongly conserved part of the protein. PMID: 16950399
    4. The role of the N-terminal domain of BipD, a type III secretion system needle tip protein, is to chaperone residues on the coiled-coil involved in interactions at the tip of the needle. PMID: 17077085
  • 亚细胞定位：
    Secreted. Note=Secreted via the bsa type III secretion system. Localizes to the tip of the external secretion needle that is part of the secretion apparatus.
  • 蛋白家族：
    Invasin protein D family
  • 数据库链接：

    KEGG: bps:BPSS1529

    STRING: 272560.BPSS1529