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  4. Recombinant Bovine Pulmonary surfactant-associated protein B (SFTPB)

Recombinant Bovine Pulmonary surfactant-associated protein B (SFTPB)

  • 中文名称：
    牛SFTPB重组蛋白
  • 货号：
    CSB-YP021173BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛SFTPB重组蛋白
  • 货号：
    CSB-EP021173BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    牛SFTPB重组蛋白
  • 货号：
    CSB-EP021173BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛SFTPB重组蛋白
  • 货号：
    CSB-BP021173BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛SFTPB重组蛋白
  • 货号：
    CSB-MP021173BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    SFTPB
  • Uniprot No.：
  • 别名：
    SFTPB; SFTP3; Pulmonary surfactant-associated protein B; SP-B; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe)
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 分子量：
    22.7 kDa
  • 表达区域：
    188-266aa
  • 氨基酸序列
    FPIPIPYCWLCRTLIKRIQAVIPKGVLAMTVAQVCHVVPLLVGGICQCLVERYSVILLDTLLGRMLPQLVCGLVLRCSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am interested in the Catalog # CSB-YP021173BO SFTPB Recombinant Protein and has the following questions:
1.Does the buffer contain some detergent to preserve the structure because of its hydrophobicity?
2.Have you tested biological activity?

A:
Very nice to receive your inquiry.
1. Our default buffer is Tris-based buffer, 50% glycerol which does not contain detergent.
If the customer has special requirement for buffer, please kindly consult with us in pre-sales and remark the requirement in your PO.
2. We haven't tested the biological activity of this protein yest. The protein we provide is mature and full-length, and expressed by Eukaryotic expression system, which should have activity in theory.
However, we haven't done validation yet.
We have interest in the customer's proposal. We could offer 10ug free sample but they should pay the shipping cost.

靶点详情

  • 功能：
    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
  • 基因功能参考文献：
    1. Measurements of the depth of SP-B(CTERM) indicated the peptide center positions ~8A more deeply than the phosphate headgroups, a topology that may allow the peptide to promote functional lipid structures without causing micellization upon compression. PMID: 22252270
    2. results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface) PMID: 22037357
    3. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
    4. Reactive oxygen species inactivation of lipid extract surfactant B (SP-B) biophysical activity appears to involve modification of a maximum of three amino acids: Trp9, Met29, and Met65. PMID: 17425286
  • 亚细胞定位：
    Secreted, extracellular space, surface film.
  • 数据库链接：

    KEGG: bta:507398

    STRING: 9913.ENSBTAP00000042549

    UniGene: Bt.42696