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  4. Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

  • 中文名称：
    牛PPT1重组蛋白
  • 货号：
    CSB-YP018587BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛PPT1重组蛋白
  • 货号：
    CSB-EP018587BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛PPT1重组蛋白
  • 货号：
    CSB-BP018587BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛PPT1重组蛋白
  • 货号：
    CSB-MP018587BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    PPT1; CLN1; PPT; Palmitoyl-protein thioesterase 1; PPT-1; EC 3.1.2.22; Palmitoyl-protein hydrolase 1
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    28-306
  • 氨基酸序列
    DPP APLPLVIWHG MGDSCCNPLS MGAIKKMVEK KIPGIHVLSL EIGKTLREDV ENSFFLNVNS QVTTVCQILA KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMVNLISVG GQHQGVFGLP RCPGESSHIC DFIRKTLNAG AYNKAIQERL VQAEYWHDPI REDIYRNHSI FLADINQERG VNESYKKNLM ALKKFVMVKF LNDTIVDPVD SEWFGFYRSG QAKETIPLQE STLYTQDRLG LKAMDKAGQL VFLALEGDHL QLSEEWFYAH IIPFLE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP018587BO, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, have any customers reported this protein to be active?

A:
Thanks for your inquiry.
Recombinant Bovine Palmitoyl-protein thioesterase 1(PPT1)
CSB-EP018587BO >> E.coli
Expression Region: 28-306aa; Full Length of Mature Protein
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

DPPAPLPLVIWHGMGDSCCNPLSMGAIKKMVEKKIPGIHVLSLEIGKTLREDVENSFFLNVNSQVTTVCQILAKDPKLQQGYNAMGFSQGGQFLRAVAQRCPSPPMVNLISVGGQHQGVFGLPRCPGESSHICDFIRKTLNAGAYNKAIQERLVQAEYWHDPIREDIYRNHSIFLADINQERGVNESYKKNLMALKKFVMVKFLNDTIVDPVDSEWFGFYRSGQAKETIPLQESTLYTQDRLGLKAMDKAGQLVFLALEGDHLQLSEEWFYAHIIPFLE


Sorry, as this is a semi-custom protein, which means we have never expressed any of these proteins before, so also haven't tested the activity or received the feedback from other customers, we can't 100% guarantee its activity.However, as what we provide is the full length of the mature protein, and it's purified under very mild condition, we recommend you to purchase a small size of eukaryotic expressed protein to have a try.
Generally, the more advanced expression system is the protein expressed with, the more likely to be active.
Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839016/
This literature introduces that the Human protein expressed by the Mammalian cell expression system is ative, while the homology between bovine and human protein is 94%. So in theory, the bovine protein should also be active.

靶点详情

  • 功能：
    Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • 亚细胞定位：
    Lysosome. Secreted.
  • 蛋白家族：
    Palmitoyl-protein thioesterase family
  • 组织特异性：
    Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle.
  • 数据库链接：

    KEGG: bta:281421

    STRING: 9913.ENSBTAP00000017780

    UniGene: Bt.5019