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  4. Recombinant Bovine Long-wave-sensitive opsin 1 (OPN1LW), partial

Recombinant Bovine Long-wave-sensitive opsin 1 (OPN1LW), partial

  • 中文名称：
    牛OPN1LW重组蛋白
  • 货号：
    CSB-YP858376BO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛OPN1LW重组蛋白
  • 货号：
    CSB-EP858376BO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    牛OPN1LW重组蛋白
  • 货号：
    CSB-EP858376BO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛OPN1LW重组蛋白
  • 货号：
    CSB-BP858376BO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛OPN1LW重组蛋白
  • 货号：
    CSB-MP858376BO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    OPN1LW; RCP; Long-wave-sensitive opsin 1; Red cone photoreceptor pigment; Red-sensitive opsin
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
  • 基因功能参考文献：
    1. an extended network of hydrogen bonds connects the agonist retinal binding site to the G protein binding site via conserved amino acid residues. dynamics of the hydrogen-bonding network inside opsin couples to interactions at the G protein binding site PMID: 25138898
    2. instability of the retinitis pigmentosa P23H mutant opsin PMID: 24515108
    3. Ligand binding in opsin apparently follows an induced-fit three-point interaction model, with the ring, 9-methyl group, and aldehyde group as the determining selection elements. PMID: 15544351
    4. the crystal structure of ligand-free native opsin from bovine retinal rod cells at 2.9 angstrom (A) resolution PMID: 18563085
    5. 3.2 A crystal structure of the bovine Ops*-GalphaCT peptide complex PMID: 18818650
    6. Mammalian rod opsin biogenesis does not appear to have an absolute requirement for Cnx. PMID: 19116670
    7. a single dibasic degron motif is sufficient to initiate both the ER retention and subsequent degradation of ospin via an endoplasmic reticulum associated degradation pathway PMID: 19878048

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Opsin subfamily
  • 组织特异性：
    Expressed in retina (at protein level). Expressed in cone and/or rod photoreceptor cells (at protein level).
  • 数据库链接：

    KEGG: bta:282293

    STRING: 9913.ENSBTAP00000027054

    UniGene: Bt.7217