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  4. Recombinant Bovine Interferon tau-1 (IFNT1)

Recombinant Bovine Interferon tau-1 (IFNT1)

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  • 中文名称：
    牛IFNT1重组蛋白
  • 货号：
    CSB-YP322795BO
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    IFNT1
  • Uniprot No.：
  • 别名：
    IFNT1; Interferon tau-1; IFN-tau-1; Antiluteolysin; Trophoblast antiluteolytic protein; Trophoblast protein 1; TP-1; Trophoblastin
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    21.8kDa
  • 表达区域：
    24-195aa
  • 氨基酸序列
    CYLSEDHMLGARENLRLLARMNRLSPHPCLQDRKDFGLPQEMVEGNQLQKDQAISVLHEMLQQCFNLFYTEHSSAAWNTTLLEQLCTGLQQQLEDLDACLGPVMGEKDSDMGRMGPILTVKKYFQGIHVYLKEKEYSDCAWEIIRVEMMRALSSSTTLQKRLRKMGGDLNSL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this Recombinant Bovine MIF protein began at the genetic level, where the coding sequence for the MIF protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Yeast) so that the cells could be cultured and expressed the desired IFNT1 protein. And we finally got the recombinant IFNT1 protein with the purity of 90%+ determined by SDS-PAGE.

    IFNT1 is a novel type of I interferon. Trophoblasts of ruminant species secrete multiple forms of IFNT1. IFNT1 is structurally related to IFN-a and IFN-β. IFNT1 are currently found only in ruminant ungulate species. There are at least three to four functional IFNT1 genes in cattle and sheep. The proteins are produced in large quantities by the trophoblast during the period of conceptus elongation that occurs just prior to initiation of implantation. Exposure of the uterus to IFNT1 appears to reduce the pulsatile release of prostaglandin. Despite this involvement in recue of the corpus luteum of pregnancy, the IFNT1 display the typical biological properties of other type I IFN. IFNT1 constitute a serologically distinct group and differ considerably in amino acid sequence from other type I IFN. Additionally, IFNT1 gene expression is poorly induced by virus. More importantly, evidence is accumulating that IFNT1 may be considerably more potent in their ability to extend inter-estrous intervals than other type I IFN in no pregnant ewes..

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Paracrine hormone primarily responsible for maternal recognition of pregnancy. Interacts with endometrial receptors, probably type I interferon receptors, and blocks estrogen receptor expression, preventing the estrogen-induced increase in oxytocin receptor expression in the endometrium. This results in the suppression of the pulsatile endometrial release of the luteolytic hormone prostaglandin F2-alpha, hindering the regression of the corpus luteum (luteolysis) and therefore a return to ovarian cyclicity. This, and a possible direct effect of IFN-tau on prostaglandin synthesis, leads in turn to continued ovarian progesterone secretion, which stimulates the secretion by the endometrium of the nutrients required for the growth of the conceptus. In summary, displays particularly high antiviral and antiproliferative potency concurrently with particular weak cytotoxicity, high antiluteolytic activity and immunomodulatory properties. In contrast with other IFNs, IFN-tau is not virally inducible.
  • 基因功能参考文献：
    1. Interferon tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus, which may provide immunological tolerance to accept the embryo. PMID: 29702095
    2. The data suggest that TEAD relocation and/or YAP degradation following its phosphorylation down-regulates IFNT gene transcription after conceptus attachment to the uterine endometrium. PMID: 27315596
    3. Report interferon-tau dependent STAT1 regulation of SOCS genes in bovine endometrium during estrus cycle and embryo implantation. PMID: 25116692
    4. Suggest the involvement of IFNT function within the corpus luteum in the establishment of pregnancy in cows. PMID: 26078085
    5. These results indicate that HB-EGF and its receptors expression changed in bovine endometrium throughout the oestrous cycle; IFN-tau increased their expression in cultured endometrial cells. PMID: 25779761
    6. Data suggest exposure to maternal CSF2 from D5-D7 of development is fundamentally different for female/male blastocysts with respect to embryo elongation, characteristics of transcriptome/methylome, and endometrial interferon tau secretion at D15. PMID: 25078682
    7. as conceptuses attach to the uterine epithelium, IFNT gene transcription is down-regulated by an increase in EOMES expression and EOMES-CREBBP binding in the attached trophoblast cells. PMID: 23606646
    8. Heat shock increased both HSP70 and IFNT expression in blastocysts. PMID: 23638875
    9. The aims of this study were to identify the earliest transcriptional response of the bovine endometrium to the presence of the conceptus and investigate if these genes are regulated by interferon tau (IFNT). PMID: 22759920
    10. Bos taurus (B. T.) Coreanae IFN-tau was cloned from peripheral blood mononuclear cells. The amino acid sequence of B. T. Coreanae IFN-tau shares only 90.3% identity with that of Holstein dairy cow. PMID: 22578803
    11. activity in the early pregnancy endometrium in cattle is modulated by cortisol PMID: 22361386
    12. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes and tumor necrosis factor-alpha mRNA levels in neutrophils and eosinophils. PMID: 22052007
    13. In contrast to previous studies in the ovine, granulocyte-macrophage colony-stimulating factor did not increase interferon tau secretion but in agreement with the ovine did not affect bovine blastocyst development. PMID: 20977503
    14. Differentially expressed genes in the endometrial transcriptome are a consequence of IFNT production by the conceptus. PMID: 21349821
    15. The increase in calving rate caused by CSF2 treatment involves, in part, more extensive development of extraembryonic membranes and capacity of the conceptus to secrete IFNT2 at day 15 of pregnancy. PMID: 21339286
    16. investigation of signaling mechanism used by fibroblast growth factor-2 (FGF2) to regulate IFNT production in trophoblasts: several lines of evidence suggest that FGF2 regulates IFNT production in trophoblasts by acting through protein kinase C-delta PMID: 21191110
    17. These data suggest that GATA2/3 is involved in trophoblast-specific regulation of bovine intereron tau gene transcription. PMID: 19598245
    18. During pregnancy, interferon-tau may block ERalpha in the luminal epithelium but likely does not rescue integrin alphavbeta3. expression. PMID: 12756058
    19. Importance of complex Ets-2 enhancer for expression of interferon-tau. Possible means whereby the mother can exert control over conceptus IFN-tau production. PMID: 15217985
    20. The increase in interferon tau concentrations responsible for the maternal recognition of pregnancy results from the increase in embryo size during elongation and not from any upregulation of mRNA expression. PMID: 16435375
    21. FGF-2 stimulates IFNT expression and plays an active role in regulating the establishment and maintenance of pregnancy in ruminants PMID: 16574787
    22. effect of IFN-tau on the proliferation and distribution of peripheral blood lymphocyte subsets during one-way mixed lymphocyte reaction (MLR) in cows and heifers PMID: 16870264
    23. abnormal gene expression of DNMT, INFT, and MHC1 was noted in the majority of cloned embryos, indicating inefficient nuclear reprogramming and retarded embryo development. PMID: 18199878
    24. DLX3 has a central role in controlling IFNT gene expression by associating with ETS2 on the IFNT promoter. PMID: 18322277
    25. Endogenous IFNT transcription in MDBK cells (which do not normally express IFNT) can be induced through CDX2 overexpression and high H3K18 acetylation. PMID: 19211809

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  • 亚细胞定位：
    Secreted. Note=Secreted into the uterine lumen.
  • 蛋白家族：
    Alpha/beta interferon family, IFN-alphaII subfamily
  • 组织特异性：
    Constitutively and exclusively expressed in the mononuclear cells of the extraembryonic trophectoderm.
  • 数据库链接：

    KEGG: bta:317698

    STRING: 9913.ENSBTAP00000047689

    UniGene: Bt.328