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  4. Recombinant Bothrops atrox Thrombin-like enzyme batroxobin

Recombinant Bothrops atrox Thrombin-like enzyme batroxobin

  • 货号：
    CSB-YP356422BUU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356422BUU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356422BUU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356422BUU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    ; Thrombin-like enzyme batroxobin; BX; SVTLE; EC 3.4.21.74; Bothrops atrox serine proteinase; Defibrase; Fibrinogen-clotting enzyme; Reptilase; Snake venom serine protease; SVSP; Venombin A
  • 种属：
    Bothrops atrox (Barba amarilla) (Fer-de-lance)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-255
  • 氨基酸序列
    VIGGDE CDINEHPFLA FMYYSPRYFC GMTLINQEWV LTAAHCNRRF MRIHLGKHAG SVANYDEVVR YPKEKFICPN KKKNVITDKD IMLIRLDRPV KNSEHIAPLS LPSNPPSVGS VCRIMGWGAI TTSEDTYPDV PHCANINLFN NTVCREAYNG LPAKTLCAGV LQGGIDTCGG DSGGPLICNG QFQGILSWGS DPCAEPRKPA FYTKVFDYLP WIQSIIAGNK TATCP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Does Cusabio provide recombinant batroxobin with validated biological activity?

A:
We recommend this protein.
Recombinant Bothrops atrox Thrombin-like enzyme batroxobin
CSB-YP356422BUU >> Yeast Lead time: 35-45 business days.
CSB-EP356422BUU >> E.coli Lead time: 35-45 business days.
CSB-BP356422BUU >> Baculovirus Lead time: 35-50 business days.
CSB-MP356422BUU >> Mammalian cell Lead time: 35-45 business days.
Expression region: 25-255aa ; full length of mature protein;
Tag information: Tag type will be determined during the manufacturing process. (Please let us know if you have any special request of tag, we will preferentially try your desired tag. If the requested tag fails, we will provide other tagged protein. )
Sequence of target protein (Sequence of tag is not included):

VIGGDECDINEHPFLAFMYYSPRYFCGMTLINQEWVLTAAHCNRRFMRIHLGKHAGSVANYDEVVRYPKEKFICPNKKKNVITDKDIMLIRLDRPVKNSEHIAPLSLPSNPPSVGSVCRIMGWGAITTSEDTYPDVPHCANINLFNNTVCREAYNGLPAKTLCAGVLQGGIDTCGGDSGGPLICNGQFQGILSWGSDPCAEPRKPAFYTKVFDYLPWIQSIIAGNKTATCP


Regarding activity
This is a semi-customized protein, we haven't tested activity of this protein, so we can't 100% guaranteed its activity. But what we provide is the full length of the mature protein, which is purified under mild condition, it should be active in theory.
Reference: https://eurekamag.com/pdf/004/004169771.pdf
It reports the cDNA encoding batroxobin was cloned, expressed in Pichia pastoris and the molecular function of purified recombinant protein was also characterized.The purified recombinant protein strongly converted fibrinogen into fibrin clot in vitro, and shortened bleeding time and whole blood coagulation time in vivo. We recommend you to order a small size to have a try.

靶点详情

  • 功能：
    Thrombin-like snake venom serine protease. Cleaves Arg-Gly bonds in fibrinogen alpha chains (FGA).
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Snake venom subfamily
  • 组织特异性：
    Expressed by the venom gland.
  • 数据库链接：

    KEGG: ag:AAA48552