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  4. Recombinant Bacillus subtilis tRNA (Ile)-lysidine synthase

Recombinant Bacillus subtilis tRNA (Ile)-lysidine synthase

  • 中文名称：
    枯草芽孢杆菌tilS重组蛋白
  • 货号：
    CSB-YP336316BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌tilS重组蛋白
  • 货号：
    CSB-EP336316BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌tilS重组蛋白
  • 货号：
    CSB-EP336316BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌tilS重组蛋白
  • 货号：
    CSB-BP336316BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌tilS重组蛋白
  • 货号：
    CSB-MP336316BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tilS
  • Uniprot No.：
  • 别名：
    tilS; yacA; BSU00670; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-472
  • 氨基酸序列
    MKSVKDFLNK HNLTLKGATI IVGVSGGPDS MALLHALHTL CGRSANVIAA HVDHRFRGAE SEEDMRFVQA YCKAEQLVCE TAQINVTAYA QEKGLNKQAA ARDCRYQFFE EIMSKHQADY LALAHHGDDQ VETMLMKLAK GTLGTGLAGM QPVRRFGTGR IIRPFLTITK EEILHYCHEN GLSYRTDESN AKDDYTRNRF RKTVLPFLKQ ESPDVHKRFQ KVSEALTEDE QFLQSLTKDE MNKVITSQSN TSVEINSSQL LALPMPLQRR GVQLILNYLY ENVPSSFSAH HIQQFLDWAE NGGPSGVLDF PKGLKVVKSY QTCLFTFEQW QCKNVPFEYQ ISGAADETAV LPNGYLIEAR HYADSPEEHG NAVFITSEKK VRFPLTIRTR KAGDRIKLKG MNGSKKVKDI FIDKKLPLQE RDNWPIVTDA SGEIIWIPGL KKSIFEDLVI PNSDRIVLQY RQHEKCRGQA KS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
  • 基因功能参考文献：
    1. Results are in agreement with rare cases of eubacteria (and archaea), which naturally lack the tilS gene (or tiaS in archaea) but contain a tRNA(Ile2) gene containing a TAT instead of a CAT anticodon PMID: 21435031
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRNA(Ile)-lysidine synthase family
  • 数据库链接：

    KEGG: bsu:BSU00670

    STRING: 224308.Bsubs1_010100000340