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  4. Recombinant Bacillus subtilis Transcriptional regulatory protein DegU (degU)

Recombinant Bacillus subtilis Transcriptional regulatory protein DegU (degU)

  • 货号：
    CSB-YP321154BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP321154BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP321154BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP321154BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP321154BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    degU
  • Uniprot No.：
  • 别名：
    degU; iep; BSU35490Transcriptional regulatory protein DegU; Protease production enhancer protein
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-229
  • 氨基酸序列
    MTKVNIVIID DHQLFREGVK RILDFEPTFE VVAEGDDGDE AARIVEHYHP DVVIMDINMP NVNGVEATKQ LVELYPESKV IILSIHDDEN YVTHALKTGA RGYLLKEMDA DTLIEAVKVV AEGGSYLHPK VTHNLVNEFR RLATSGVSAH PQHEVYPEIR RPLHILTRRE CEVLQMLADG KSNRGIGESL FISEKTVKNH VSNILQKMNV NDRTQAVVVA IKNGWVEMR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase. Involved in the control of expression of different cellular functions, including production of degradative enzymes such as the neutral and alkaline proteases, flagellum formation, biofilm formation, and competence for DNA uptake. Positively or negatively regulates expression of many different genes. The phosphorylated form is required for synthesis of degradative enzymes, flagellum formation and biofilm formation. The unphosphorylated form is required for expression of genetic competence, via induction of comK.
  • 基因功能参考文献：
    1. Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis. PMID: 25880922
    2. The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. PMID: 24386445
    3. An electrophoretic mobility shift assay (EMSA) showed that SinR bound to the degU promoter and that SlrR formed a complex with SinR on the degU promoter. PMID: 24317403
    4. High levels of phosphorylated DegU activate an Esat-6-like secretion system in Bacillus subtilis. PMID: 23861817
    5. Western blot analysis of DegU in disruption mutants of genes encoding various ATP-dependent proteases strongly suggested that ClpCP degrades DegU. PMID: 20070525
    6. Results show that gradual increase in DegU and phospho-DegU levels induces a transition from growth as motile cells to growth as sessile, biofilm-forming cells. PMID: 17850253
    7. The phosphorylated form of DegU is known to activate transcription of more than 120 genes in B. subtilis, including the bpr gene encoding bacillopeptidase F. PMID: 18194340
    8. These results suggest that degU expression is controlled by the three promoters under different growth conditions. PMID: 18502860
    9. six-base deletion within the sequence (the -44 to -39 region) abolished DegU-binding to the pgsB promoter and pgsB transcription, confirming the importance of the sequence for DegU-dependent regulation of pgsB PMID: 19734658

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: bsu:BSU35490

    STRING: 224308.Bsubs1_010100019196