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  4. Recombinant Bacillus subtilis Stage IV sporulation protein A (spoIVA)

Recombinant Bacillus subtilis Stage IV sporulation protein A (spoIVA)

  • 货号：
    CSB-YP336020BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP336020BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP336020BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP336020BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP336020BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    spoIVA
  • Uniprot No.：
  • 别名：
    spoIVA; spoVP; BSU22800; Stage IV sporulation protein A; EC 3.6.1.3; Coat morphogenetic protein SpoIVA
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-492
  • 氨基酸序列
    MEKVDIFKDI AERTGGDIYL GVVGAVRTGK STFIKKFMEL VVLPNISNEA DRARAQDELP QSAAGKTIMT TEPKFVPNQA MSVHVSDGLD VNIRLVDCVG YTVPGAKGYE DENGPRMINT PWYEEPIPFH EAAEIGTRKV IQEHSTIGVV ITTDGTIGDI ARSDYIEAEE RVIEELKEVG KPFIMVINSV RPYHPETEAM RQDLSEKYDI PVLAMSVESM RESDVLSVLR EALYEFPVLE VNVNLPSWVM VLKENHWLRE SYQESVKETV KDIKRLRDVD RVVGQFSEFE FIESAGLAGI ELGQGVAEID LYAPDHLYDQ ILKEVVGVEI RGRDHLLELM QDFAHAKTEY DQVSDALKMV KQTGYGIAAP ALADMSLDEP EIIRQGSRFG VRLKAVAPSI HMIKVDVESE FAPIIGTEKQ SEELVRYLMQ DFEDDPLSIW NSDIFGRSLS SIVREGIQAK LSLMPENARY KLKETLERII NEGSGGLIAI IL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATPase. Has a role at an early stage in the morphogenesis of the spore coat outer layers. Its ATP hydrolysis is required for proper assembly of the spore coat. Forms a basement layer around the outside surface of the forespore and self-assembles irreversibly into higher order structures by binding and hydrolyzing ATP thus creating a durable and stable platform upon which thereafter morphogenesis of the coat can take place. Required for proper localization of spore coat protein CotE and sporulation-specific proteins including SpoVM.
  • 基因功能参考文献：
    1. s propose a model in which SpoIVA initially assumes a conformation in which it inhibits its own aggregation into particles, and that ATP hydrolysis remodels the protein so that it assumes a polymerization-competent conformation. PMID: 24810258
    2. Once SpoIVA is recruited to the surface of the developing spore, it uses ATP hydrolysis to self-assemble into a static polymer. PMID: 23267091
  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: bsu:BSU22800

    STRING: 224308.Bsubs1_010100012526