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  4. Recombinant Bacillus subtilis Signal transduction histidine-protein kinase/phosphatase DegS (degS)

Recombinant Bacillus subtilis Signal transduction histidine-protein kinase/phosphatase DegS (degS)

  • 货号：
    CSB-YP320275BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP320275BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP320275BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP320275BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    degS
  • Uniprot No.：
  • 别名：
    degS; sacU; BSU35500Signal transduction histidine-protein kinase/phosphatase DegS; EC 2.7.13.3; EC 3.1.3.-
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-385
  • 氨基酸序列
    MNKTKMDSKV LDSILMKMLK TVDGSKDEVF QIGEQSRQQY EQLVEELKQI KQQVYEVIEL GDKLEVQTRH ARNRLSEVSR NFHRFSEEEI RNAYEKAHKL QVELTMIQQR EKQLRERRDD LERRLLGLQE IIERSESLVS QITVVLNYLN QDLREVGLLL ADAQAKQDFG LRIIEAQEEE RKRVSREIHD GPAQMLANVM MRSELIERIF RDRGAEDGFQ EIKNLRQNVR NALYEVRRII YDLRPMALDD LGLIPTLRKY LYTTEEYNGK VKIHFQCIGE TEDQRLAPQF EVALFRLAQE AVSNALKHSE SEEITVKVEI TKDFVILMIK DNGKGFDLKE AKEKKNKSFG LLGMKERVDL LEGTMTIDSK IGLGTFIMIK VPLSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase. Involved in the control of expression of different cellular functions, including production of degradative enzymes such as the neutral and alkaline proteases, flagellum formation and biofilm formation. Acts as both a protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to DegU, and a protein phosphatase that dephosphorylates phospho-DegU.
  • 基因功能参考文献：
    1. A link between DegS phosphorylation at serine 76 and the level of DegU phosphorylation, establishing this post-translational modification as an additional trigger for this two-component system. PMID: 21304896
    2. Results show that degS mutation did not affect transcription of the flgB-sigD operon, but reduced transcription of sigma(D)-dependent flagellar genes, degU and other DegU-regulated genes. PMID: 17850253
  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: bsu:BSU35500

    STRING: 224308.Bsubs1_010100019201