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  4. Recombinant Bacillus subtilis Serine/threonine-protein kinase PrkC (prkC), partial

Recombinant Bacillus subtilis Serine/threonine-protein kinase PrkC (prkC), partial

  • 中文名称：
    枯草芽孢杆菌prkC重组蛋白
  • 货号：
    CSB-YP521962BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌prkC重组蛋白
  • 货号：
    CSB-EP521962BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌prkC重组蛋白
  • 货号：
    CSB-EP521962BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌prkC重组蛋白
  • 货号：
    CSB-BP521962BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌prkC重组蛋白
  • 货号：
    CSB-MP521962BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    prkC
  • Uniprot No.：
  • 别名：
    prkC; yloP; BSU15770; Serine/threonine-protein kinase PrkC; Ser/Thr-protein kinase PrkC; EC 2.7.11.1
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase that is responsible for triggering spore germination in response to muropeptides, signaling bacteria to exit dormancy. PrkC is thus a germination receptor that binds peptidoglycan fragments containing m-Dpm (meso-diaminopimelate), which act as spore germinants. Autophosphorylates and phosphorylates EF-G (elongation factor G, fusA); the latter modification is likely necessary for germination in response to peptidoglycan. Another group did not detect phosphorylation of EF-G. PrkC is a substrate in vitro of the cotranscribed phosphatase PrpC, which suggests that they form a functional couple in vivo. Might also be involved in sporulation and biofilm formation. Does not seem to be involved in stress response.
  • 基因功能参考文献：
    1. conclude that PrkC activation is not as straightforward as previously suggested and that regulation of its kinase activity via the PASTA domains and peptidoglycan fragments binding occurs when PrkC is not concentrated to the bacterial septum, but all over the cell wall in non-dividing bacillus cells. PMID: 29374241
    2. findings show that GpsB interacts with the catalytic subunit of PrkC that, in turn, phosphorylates GpsB; study concludes that phosphorylation of GpsB regulates PrkC activity through a negative feedback loop PMID: 25845974
  • 亚细胞定位：
    Spore membrane; Single-pass type II membrane protein. Note=Is associated with the inner membrane of the spore.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: bsu:BSU15770

    STRING: 224308.Bsubs1_010100008706