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  4. Recombinant Bacillus subtilis Replication termination protein (rtp)

Recombinant Bacillus subtilis Replication termination protein (rtp)

  • 中文名称：
    枯草芽孢杆菌rtp重组蛋白
  • 货号：
    CSB-YP317211BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌rtp重组蛋白
  • 货号：
    CSB-EP317211BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌rtp重组蛋白
  • 货号：
    CSB-EP317211BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌rtp重组蛋白
  • 货号：
    CSB-BP317211BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌rtp重组蛋白
  • 货号：
    CSB-MP317211BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rtp
  • Uniprot No.：
  • 别名：
    rtp; BSU18490; Replication termination protein; Replication terminator protein
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-122
  • 氨基酸序列
    MKEEKRSSTG FLVKQRAFLK LYMITMTEQE RLYGLKLLEV LRSEFKEIGF KPNHTEVYRS LHELLDDGIL KQIKVKKEGA KLQEVVLYQF KDYEAAKLYK KQLKVELDRC KKLIEKALSD NF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in DNA replication and termination (fork arrest mechanism). Two dimers of rtp bind to the two inverted repeat regions (IRI and IRII) present in the termination site. The binding of each dimer is centered on an 8 bp direct repeat.
  • 基因功能参考文献：
    1. NMR analysis of interaction of the replication terminator protein of Bacillus subtilis with DNA PMID: 16061201
    2. RTP interacts with B. subtilis replication machinery in a manner that directly contributes to the fork arrest mechanism. PMID: 16822523
    3. The functional terminator complex comprises two homodimers each of 29 kDa bound to overlapping binding sites. PMID: 17077489
    4. Results report the crystal structure of a replication terminator protein homologue (RTP.C110S) of B. subtilis in complex with the high affinity component of one of its cognate DNA termination sites, known as the TerI B-site, refined at 2.5 A resolution. PMID: 17521668
  • 数据库链接：

    KEGG: bsu:BSU18490

    STRING: 224308.Bsubs1_010100010186