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  4. Recombinant Bacillus subtilis RNA polymerase sigma-B factor (sigB)

Recombinant Bacillus subtilis RNA polymerase sigma-B factor (sigB)

  • 中文名称：
    枯草芽孢杆菌sigB重组蛋白
  • 货号：
    CSB-YP361879BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌sigB重组蛋白
  • 货号：
    CSB-EP361879BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌sigB重组蛋白
  • 货号：
    CSB-EP361879BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌sigB重组蛋白
  • 货号：
    CSB-BP361879BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌sigB重组蛋白
  • 货号：
    CSB-MP361879BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sigB
  • Uniprot No.：
  • 别名：
    sigB; rpoF; BSU04730; RNA polymerase sigma-B factor; General stress protein 84; GSP84; Sigma-37
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-262
  • 氨基酸序列
    TQPSKTTKL TKDEVDRLIS DYQTKQDEQA QETLVRVYTN LVDMLAKKYS KGKSFHEDLR QVGMIGLLGA IKRYDPVVGK SFEAFAIPTI IGEIKRFLRD KTWSVHVPRR IKELGPRIKM AVDQLTTETQ RSPKVEEIAE FLDVSEEEVL ETMEMGKSYQ ALSVDHSIEA DSDGSTVTIL DIVGSQEDGY ERVNQQLMLQ SVLHVLSDRE KQIIDLTYIQ NKSQKETGDI LGISQMHVSR LQRKAVKKLR EALIEDPSME LM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcribed in the early stationary phase under conditions inimical to sporulation. May play a role in the ability of the bacterium to adapt to various stresses but is not essential for its survival under these conditions. Positively regulates expression of its own operon. The second most abundant sigma factor, it associates with RNAP core under all growth phases.
  • 基因功能参考文献：
    1. The reduction in small ribosomal subunit abundance in ethanol-stressed cells of Bacillus subtilis is mediated by a SigB-dependent antisense RNA PMID: 26115952
    2. In contrast to the -10 element binding specificity observed for B. subtilis sigma(D) , the promoter binding of sigma(B) is specific for the -35 element. PMID: 25652417
    3. Ectopic expression of sigma(B) and subsequent induction of spo0E cause a sigma(B)-dependent block of sporulation-specific transcription of the spo0A and spoIIE genes and produces a sporulation-deficient phenotype. PMID: 22210769
  • 蛋白家族：
    Sigma-70 factor family, SigB subfamily
  • 数据库链接：

    KEGG: bsu:BSU04730

    STRING: 224308.Bsubs1_010100002673